Genetic Diversity in Cultivars of Phaseolus vulgaris L.by using Biochemical Markers.

SDS-PAGE was used to evaluate and characterize the patterns of seed storage proteins in eight cultivars of Common bean.Electrophorogram for each variety was scored for presence and absence of protein band, which was transformed into a binary data matrix.Jaccards similarity coefficient, generated to determine the genetic similarity among cultivars, it revealed that most of the cultivars were genetically distant from each other. .Eight cultivars of Phaseolus vulgaris L. were investigated for genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on seed proteins, eleven maximum band were observed in B4 (Beans French yellow )and three minimum number of protein bands in B1 (BB) .The highest similarity index was observed in cultivar B2(Surbhi) 0.909 and lowest in B4(Beans French Yellow) and B6(Laffa) 0.272 in both cultivars .The highest dissimilarity value found in B4 and B6 0.728 in both the cultivars and lowest value in B2 cultivar 0.09 The un-weighted paired group method with arithmetic averages (UPGMA) exhibited two clusters A and B which were grouped into four sub-clusters I,II,III and IV. In broader spectrum, the genotypes from various sourcesdiffered in grouping and it was difficult to establish relationship between origin and cluster pattern. Particular clusters were better for specific traits that are suggested to utilize in crop improvement program. It is concluded that SDS-PAGE analysis of common bean seed storage protein is useful for evaluation of genetic variability and cultivars identification that help in common bean breeding program.