1、2型糖尿病足溃疡病原菌的微生物学检测

Murtada Hafedh
{"title":"1、2型糖尿病足溃疡病原菌的微生物学检测","authors":"Murtada Hafedh","doi":"10.32792/utq/utjsci/v10i1.1042","DOIUrl":null,"url":null,"abstract":"Globally, diabetes mellitus (DM) is a serious health issue. Diabetic neuropathy, the most prevalent micro-vascular consequence of diabetes, raises the chance of developing foot ulcers. Different aerobic bacteria, including Gram positive cocci, Staphylococcus aureus, and (beta-haemolytic) Streptococcus are some of the pathogens that can colonize DFUs. Coagulation-negative Staphylococcus (CNS), Corynebacterium, Enterococcus, and Cutibacteria are frequently found in ulcer cultures in clinical practice; however they are typically regarded as colonizing rather than harmful bacteria. Anaerobes are detected in a smaller minority of DFIs and have been associated to altered innate and adaptive immune responses in the hyperglycemic milieu. Gram-negative bacteria account for about one third of DFIs. About 70 participants in all, including 29 individuals with type 1 diabetes and 41 patients with type 2 diabetes who have diabetic foot ulcers. Scarping of ulcers takes place after regular saline wound cleansing to eliminate surface pollutants, and then exudate is sampled. Pus, or discharges from the base of the ulcer, are then submitted to the laboratory as quickly as possible using an aseptic approach. Then, the bacteria were inoculated onto various culture media, such as MacConkey and Blood agar as enrichment media, and incubated aerobically at 37°C for 24 hours to isolate aerobic bacteria. The following day, the bacteria were checked for growth, and a pure colony was then prepared. According to morphological, microscopic, and particular cards of the automated VITEK2 system, isolated bacteria were identified.","PeriodicalId":23432,"journal":{"name":"University of Thi-Qar Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microbiological Detection of Pathogenic Bacteria in Diabetic Foot Ulcers of Type 1 and 2 Diabetic Mellitus Patients\",\"authors\":\"Murtada Hafedh\",\"doi\":\"10.32792/utq/utjsci/v10i1.1042\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Globally, diabetes mellitus (DM) is a serious health issue. Diabetic neuropathy, the most prevalent micro-vascular consequence of diabetes, raises the chance of developing foot ulcers. Different aerobic bacteria, including Gram positive cocci, Staphylococcus aureus, and (beta-haemolytic) Streptococcus are some of the pathogens that can colonize DFUs. Coagulation-negative Staphylococcus (CNS), Corynebacterium, Enterococcus, and Cutibacteria are frequently found in ulcer cultures in clinical practice; however they are typically regarded as colonizing rather than harmful bacteria. Anaerobes are detected in a smaller minority of DFIs and have been associated to altered innate and adaptive immune responses in the hyperglycemic milieu. Gram-negative bacteria account for about one third of DFIs. About 70 participants in all, including 29 individuals with type 1 diabetes and 41 patients with type 2 diabetes who have diabetic foot ulcers. Scarping of ulcers takes place after regular saline wound cleansing to eliminate surface pollutants, and then exudate is sampled. Pus, or discharges from the base of the ulcer, are then submitted to the laboratory as quickly as possible using an aseptic approach. Then, the bacteria were inoculated onto various culture media, such as MacConkey and Blood agar as enrichment media, and incubated aerobically at 37°C for 24 hours to isolate aerobic bacteria. The following day, the bacteria were checked for growth, and a pure colony was then prepared. According to morphological, microscopic, and particular cards of the automated VITEK2 system, isolated bacteria were identified.\",\"PeriodicalId\":23432,\"journal\":{\"name\":\"University of Thi-Qar Journal of Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"University of Thi-Qar Journal of Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32792/utq/utjsci/v10i1.1042\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"University of Thi-Qar Journal of Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32792/utq/utjsci/v10i1.1042","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

全球范围内,糖尿病(DM)是一个严重的健康问题。糖尿病神经病变,糖尿病最普遍的微血管后果,增加了发展足溃疡的机会。不同的需氧细菌,包括革兰氏阳性球菌、金黄色葡萄球菌和(溶血)链球菌是一些可以定植dfu的病原体。凝固阴性葡萄球菌(CNS)、棒状杆菌、肠球菌和表皮细菌在临床溃疡培养中经常被发现;然而,它们通常被认为是定植菌而不是有害细菌。在少数dfi患者中检测到厌氧菌,并且与高血糖环境中先天和适应性免疫反应的改变有关。革兰氏阴性菌约占dfi的三分之一。总共约70名参与者,包括29名1型糖尿病患者和41名患有糖尿病足溃疡的2型糖尿病患者。在常规生理盐水清洗伤口以消除表面污染物后,将溃疡划破,然后对渗出物进行取样。脓液,或溃疡底部的分泌物,然后用无菌方法尽快送到实验室。然后,将细菌接种于各种培养基上,如以MacConkey和Blood琼脂为富集培养基,37℃好氧培养24小时,分离好氧菌。第二天,检查细菌的生长情况,然后准备一个纯菌落。根据自动VITEK2系统的形态学,显微和特定卡片,鉴定分离的细菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiological Detection of Pathogenic Bacteria in Diabetic Foot Ulcers of Type 1 and 2 Diabetic Mellitus Patients
Globally, diabetes mellitus (DM) is a serious health issue. Diabetic neuropathy, the most prevalent micro-vascular consequence of diabetes, raises the chance of developing foot ulcers. Different aerobic bacteria, including Gram positive cocci, Staphylococcus aureus, and (beta-haemolytic) Streptococcus are some of the pathogens that can colonize DFUs. Coagulation-negative Staphylococcus (CNS), Corynebacterium, Enterococcus, and Cutibacteria are frequently found in ulcer cultures in clinical practice; however they are typically regarded as colonizing rather than harmful bacteria. Anaerobes are detected in a smaller minority of DFIs and have been associated to altered innate and adaptive immune responses in the hyperglycemic milieu. Gram-negative bacteria account for about one third of DFIs. About 70 participants in all, including 29 individuals with type 1 diabetes and 41 patients with type 2 diabetes who have diabetic foot ulcers. Scarping of ulcers takes place after regular saline wound cleansing to eliminate surface pollutants, and then exudate is sampled. Pus, or discharges from the base of the ulcer, are then submitted to the laboratory as quickly as possible using an aseptic approach. Then, the bacteria were inoculated onto various culture media, such as MacConkey and Blood agar as enrichment media, and incubated aerobically at 37°C for 24 hours to isolate aerobic bacteria. The following day, the bacteria were checked for growth, and a pure colony was then prepared. According to morphological, microscopic, and particular cards of the automated VITEK2 system, isolated bacteria were identified.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信