茶树内生真菌的生物防治潜力(Kuntze)叶片抗选定的真菌植物病原体

IF 0.4 Q4 MICROBIOLOGY

Malaysian Journal of Microbiology Pub Date : 2022-12-01 DOI:10.21161/mjm.220055

Kasun M. Thambugala, D. Daranagama, S. Kannangara

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引用次数: 1

摘要

目的:内生真菌是一组多样的微生物，在宿主的健康组织中无症状地停留。许多真菌内生菌与茶树(Camellia sinensis)有关，茶树的病原体在植物的初始生长过程中有可能作为内生菌生长或作为潜伏病原体。本研究旨在筛选具有抑制几种重要植物病原体镰刀菌、茶叶枯plodia、拟盘多毛菌和菌核菌潜力的茶叶内生真菌，并通过体外实验评价内生真菌分离物对这些植物病原体的抑制效果。方法与结果:5株内生真菌;研究了从斯里兰卡产的中国紫杉叶片中分离得到的炭疽菌、暹罗炭疽菌、绿绿达尔丁菌、中国伪拟青竹菌和资本毛缕菌对植物病原菌的生长抑制作用;镰刀菌属、可可菌属、拟盘多毛菌属和镰刀菌属。利用双重培养试验和挥发性化合物介导的抑制试验。本研究使用的真菌内生菌均对镰刀菌、拟盘多毛菌和拟盘多毛菌具有抗真菌活性。在PDA上进行双培养试验。Daldinia eschscholtzii(67.30%)和C. gloeosporioides(61.54%)对菌核病菌表现出较强的拮抗活性，而P. capitalensis(42.30%)表现出中等拮抗活性。所有内生真菌菌株对镰刀菌的抑菌活性均为中等，对拟盘多毛孢的抑菌率均在25.00%以下。在挥发性化合物介导的抑制实验中，没有一种内生菌株对所使用的植物病原体有明显的抑制作用。结论、研究意义及影响:本研究发现对植物病原菌具有潜在拮抗活性的真菌内生菌，可用于开发真菌生物防治剂(BCAs)的商业化产品，防治植物真菌病害。

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Biocontrol potential of endophytic fungi in tea (Camellia sinensis (L.) Kuntze) leaves against selected fungal phytopathogens

Aims: Endophytic fungi are a diverse group of microorganisms that stay asymptomatically in the healthy tissues of the host. Many fungal endophytes are associated with the tea plant ( Camellia sinensis ) and the pathogens of the tea plant have the potential to grow as endophytes or act as latent pathogens during the initial growth of the plant. The present study aimed at screening tea endophytic fungi with the potential for control of a few critical phytopathogens, Fusarium sp., Lasiodiplodia theobromae , Pestalotiopsis sp. and Sclerotinia sclerotiorum while evaluating the efficiency of growth inhibition of these phytopathogens by the endophytic fungal isolates using in vitro assays. Methodology and results: Five endophytic fungal strains; Colletotrichum gloeosporioides , C. siamense , Daldinia eschscholtzii , Pseudopestalotiopsis chinensis and Phyllosticta capitalensis isolated from leaves of C. sinensis in Sri Lanka were evaluated for growth inhibition against plant pathogens; Fusarium sp., L. theobromae , Pestalotiopsis sp. and S . sclerotiorum using the dual culture assay and volatile compound-mediated inhibition assay. All the fungal endophytes used in this study exhibited antifungal activity against Fusarium sp., Pestalotiopsis sp. and S . sclerotiorum in the dual culture assay on PDA. Daldinia eschscholtzii (67.30%) and C. gloeosporioides (61.54%) showed strong antagonistic activity against S. sclerotiorum , while P. capitalensis (42.30%) demonstrated moderate activity. All the endophytic fungal strains showed moderate antifungal activities against Fusarium sp. The percentage growth inhibitions of Pestalotiopsis sp. by all the endophytic isolates tested were below 25.00%. In the volatile compound-mediated inhibition assay, none of the endophytic isolates showed visible inhibition against the phytopathogens used. Conclusion, significance and impact of study: In this study, the fungal endophytes which showed potential antagonistic activity against the tested phytopathogens can be used to develop commercialized products of fungal biocontrol agents (BCAs) for controlling plant fungal diseases.

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来源期刊

Malaysian Journal of Microbiology MICROBIOLOGY-

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： The Malaysian Journal of Microbiology (MJM) publishes high quality microbiology research related to the tropics. These include infectious diseases and antimicrobials. In addition, the journal also publishes research works on the application of microbes for the betterment of human society and the environment. The journal welcomes papers on isolation, identification, characterization and application of microbes and microbial products. The MJM is published under the auspices of the Malaysian Society for Microbiology. It serves as a forum for scientific communication among scientists and academics who deal with microbes and microbial products. The journal publishes research articles, short communications and review articles on various novel aspects of microbiology, which include topics related to medical, pharmaceutical, food, agricultural, industry, plant pathology, biotechnology, microbial genetics, environment, soil, water and biodeterioration. The journal aspires to emphasize the important roles played by microbes in our daily life.