荧光标记的Morpholino核苷三磷酸衍生物的有效合成

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2016-01-02 DOI:10.1080/15257770.2015.1114125

Y. Tarasenko, T. Abramova, Viktor I Mamatuk, V. Silnikov

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引用次数: 10

摘要

合成了具有活性官能团基团的三磷酸Morpholino核苷(A, U, G, C, T)及其荧光标记衍生物。所有化合物均通过1H, 13C, 31P NMR和质谱进行了表征。讨论了在DNA测序中使用荧光标记的morpholino核苷三磷酸作为链终止物的可能性。

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Effective Synthesis of Fluorescently Labeled Morpholino Nucleoside Triphosphate Derivatives

ABSTRACT Morpholino nucleoside triphosphates (A, U, G, C, T) bearing the active functional amino group tethered to morpholine residue and their fluorescently labeled derivatives were synthesized. All compounds were characterized by 1H, 13C, and 31P NMR, and mass spectrometry. A possibility of using fluorescently labeled morpholino nucleoside triphosphates as chain terminators in DNA sequencing is discussed.

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Nucleosides, Nucleotides and Nucleic Acids

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