Xiang-kan Zhou, Min Di, Lifen Zhang, Xueli Du, Hua-Fu Zhao, Y. Zhong, Ying-Hui Shang, Xiaochun Zhou
{"title":"雪莲培养细胞提取物抗急性低压缺氧的机制研究","authors":"Xiang-kan Zhou, Min Di, Lifen Zhang, Xueli Du, Hua-Fu Zhao, Y. Zhong, Ying-Hui Shang, Xiaochun Zhou","doi":"10.3760/CMA.J.ISSN.1007-6239.2015.01.002","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the resistance to acute hypobaric hypoxia of Saussurea involucrata cultured cell extracts and the underlying mechanism. \n \n \nMethods \nThirty-six Wistar rats were averagely divided into blank control group, negative control group and Saussurea involucrate cultured cell extracts group according to random number table. The Saussurea involucrate cultured cell extracts were infused to the rats of Saussurea involucrate cultured cell extracts group with the dose of 500 mg/kg for 14 d while the distilled water was fed to other 2 groups in the period. Except blank control group, the rats of other groups were exposed in simulative 8 000 m altitude experiencing acute hypobaric hypoxia after 14 d infusion. Then the rats of all groups were killed and sampled blood serum and brain tissue. The changes of various biochemical factors in brain tissue and blood serum, such as glutamic acid (GLU), lactic acid (LD), lactate dehydrogenase (LDH), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and glutathion peroxidase (GSH-PX) were tested. \n \n \nResults \nComparing with those of blank control group, the GLU, NO, LD, LDH and MDA in blood serum and brain tissue of negative control group were significantly increased (P<0.01), and Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX were significantly decreased (P<0.01). The GLU, NO, LD, LDH and MDA of Saussurea involucrate cultured cell extracts group were significantly lower than those of negative control group (P<0.01) but with significantly higher Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX (P<0.01). \n \n \nConclusions \nFor simulated 8 000 m hypoxia exposure, Saussurea involucrata cultured cell extracts can effectively remove or inhibit the generation of excessive oxygen free radicals and may prevent glutamic acid and lactic acid from accumulating in rat brain tissue and blood serum. By protecting the function of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase on cell membrane and preventing the consequent toxic reaction induced by energy metabolism disorder, they can alleviate cell edema, protect the integrity and function of brain tissues and cells and effectively ease acute hypobaric hypoxia. \n \n \nKey words: \nRats, Wistar; Cells, cultured; Cell extracts; Anoxia; Saussurea involucrate","PeriodicalId":9904,"journal":{"name":"中华航空航天医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanism research for the resistant effects of Saussurea involucrate cultured cell extracts on acute hypobaric hypoxia\",\"authors\":\"Xiang-kan Zhou, Min Di, Lifen Zhang, Xueli Du, Hua-Fu Zhao, Y. 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The changes of various biochemical factors in brain tissue and blood serum, such as glutamic acid (GLU), lactic acid (LD), lactate dehydrogenase (LDH), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and glutathion peroxidase (GSH-PX) were tested. \\n \\n \\nResults \\nComparing with those of blank control group, the GLU, NO, LD, LDH and MDA in blood serum and brain tissue of negative control group were significantly increased (P<0.01), and Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX were significantly decreased (P<0.01). The GLU, NO, LD, LDH and MDA of Saussurea involucrate cultured cell extracts group were significantly lower than those of negative control group (P<0.01) but with significantly higher Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX (P<0.01). \\n \\n \\nConclusions \\nFor simulated 8 000 m hypoxia exposure, Saussurea involucrata cultured cell extracts can effectively remove or inhibit the generation of excessive oxygen free radicals and may prevent glutamic acid and lactic acid from accumulating in rat brain tissue and blood serum. 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Mechanism research for the resistant effects of Saussurea involucrate cultured cell extracts on acute hypobaric hypoxia
Objective
To investigate the resistance to acute hypobaric hypoxia of Saussurea involucrata cultured cell extracts and the underlying mechanism.
Methods
Thirty-six Wistar rats were averagely divided into blank control group, negative control group and Saussurea involucrate cultured cell extracts group according to random number table. The Saussurea involucrate cultured cell extracts were infused to the rats of Saussurea involucrate cultured cell extracts group with the dose of 500 mg/kg for 14 d while the distilled water was fed to other 2 groups in the period. Except blank control group, the rats of other groups were exposed in simulative 8 000 m altitude experiencing acute hypobaric hypoxia after 14 d infusion. Then the rats of all groups were killed and sampled blood serum and brain tissue. The changes of various biochemical factors in brain tissue and blood serum, such as glutamic acid (GLU), lactic acid (LD), lactate dehydrogenase (LDH), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and glutathion peroxidase (GSH-PX) were tested.
Results
Comparing with those of blank control group, the GLU, NO, LD, LDH and MDA in blood serum and brain tissue of negative control group were significantly increased (P<0.01), and Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX were significantly decreased (P<0.01). The GLU, NO, LD, LDH and MDA of Saussurea involucrate cultured cell extracts group were significantly lower than those of negative control group (P<0.01) but with significantly higher Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, T-AOC, SOD and GSH-PX (P<0.01).
Conclusions
For simulated 8 000 m hypoxia exposure, Saussurea involucrata cultured cell extracts can effectively remove or inhibit the generation of excessive oxygen free radicals and may prevent glutamic acid and lactic acid from accumulating in rat brain tissue and blood serum. By protecting the function of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase on cell membrane and preventing the consequent toxic reaction induced by energy metabolism disorder, they can alleviate cell edema, protect the integrity and function of brain tissues and cells and effectively ease acute hypobaric hypoxia.
Key words:
Rats, Wistar; Cells, cultured; Cell extracts; Anoxia; Saussurea involucrate
期刊介绍:
The aim of Chinese Journal of Aerospace Medicine is to combine theory and practice, improve and popularize, actively advocate a hundred flowers bloom and a hundred schools of thought contend, advocate seeking truth from facts, promote the development of the related disciplines of aerospace medicine and human efficiency, and promote the exchange and penetration of aerospace medicine and human efficiency with other biomedical and engineering specialties.
Topics of interest for Chinese Journal of Aerospace Medicine include:
-The content of the journal belongs to the discipline of special medicine and military medicine, with the characteristics of multidisciplinary synthesis and cross-penetration, and mainly reflected in the aerospace industry, aerospace flight safety and efficiency, as well as the synthesis of special medicine, preventive medicine, environmental medicine, psychology, etc.
-Military aeromedicine (Air Force, Navy and Army aeromedicine) and civil aeromedicine, with a balance of aerospace medicine are the strengths of the journal.
-The change in aerospace medicine from a focus on promoting physiological compensatory adaptations to enhancing human performance under extreme environmental conditions is what the journal is helping to promote.
-The expansion of manuscripts in high altitude medicine is also a special emphasis of the journal.