龙眼蜂蜜对大鼠卵巢玻璃化后腔前卵泡完整性的冷冻保护作用。

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-01-01 DOI:10.54680/fr23110110712

N. Anita, EmptyYN Y Abinawanto, A. Jusuf, A. Bowolaksono, H. A. Saoemi, A. Safrina

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引用次数: 1

摘要

背景:龙眼蜂蜜(LH)有潜力作为一种天然的细胞外冷冻保护剂，在卵巢玻璃化过程中保持完整的腔前卵泡的完整性，防止冷冻损伤。目的探讨甘草蜂蜜对大鼠卵巢玻璃化后腔前卵泡完整性的冷冻保护作用。材料与方法玻璃化后，用石蜡法观察卵巢组织切片，苏木精-伊红染色，测定卵泡指数，Optilab 3.0和Image Raster软件分析。结果乙二醇(EG)与LH联合用药，剂量变化7.5% ~ 15% (kk1 - kp4)可增加G2和G3的卵泡密度和完整卵泡数量，G1期减少闭锁卵泡的效果优于单独用药(KKP1-KKP2)。腔前卵泡组织学结构的差异影响了每种卵泡维持卵泡结构免受低温损伤完整性所需的LH剂量。G2总卵泡指数的比较值分别为KKP1(90.7±18.3)、KKP2(115.6±9.9)< KP1(193.6±10.7)、KP2(189.3±10.5)、KP3(182.2±27.1)、KP4(169.4±8.8)。在LH剂量7.5% ~ 15%的变化中，G3指数值升高幅度最大的是初级(51.7±9.8)、第三期(43.1±8.8)、次级(33.9±4.7)和初级(28.7±2.5)的KP3卵泡(LH的7.5%)。结论大鼠卵巢玻璃化后，初级和第三期卵泡保持了最好的完整性，可用于卵巢玻璃化。doi.org/10.54680/fr23110110712。

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Cryoprotective effects of longan honey on preantral follicle integrity of rat ovary post vitrification.

BACKGROUND Longan honey (LH) has the potential as a natural extracellular cryoprotectant to maintain the integrity of intact preantral follicles against the cryoinjury during ovarian vitrification. OBJECTIVE This research determined the cryoprotective effects of logan honey on preantral follicles integrity of rat ovary post-vitrification. MATERIALS AND METHODS After vitrification, the follicle index was determined by observing the ovarian histological sections made using the paraffin method with hematoxylin-eosin staining and analyzed using Optilab 3.0 and Image Raster software. RESULTS The results showed that the combination of ethylene glycol (EG) with LH and a dose variation of 7.5 %-15 % (KP1-KP4) increased the density of follicles, the number of intact follicles in G2 and G3, with a decrease in the atretic follicles in G1 better compared to the use of EG only (KKP1-KKP2). The differences in the histological structur e of preantral follicles affected the doses of LH needed by each type of follicle to maintain the integrity of the follicular structure from cryoinjury effects. The comparison of the G2 total follicle index values were KKP1 (90.7 ± 18.3), KKP2 (115.6 ± 9.9) < KP1 (193.6 ± 10.7), KP2 (189.3 ± 10.5), KP3 (182.2 ± 27.1) and KP4 (169.4±8.8). Among the variations in the dose of LH 7.5 % - 15%, the highest increase in the G3 index value was found in primary (51.7 ± 9.8), tertiary (43.1 ± 8.8), secondary (33.9 ± 4.7), and primordial (28.7 ± 2.5) follicles of KP3 (7.5 % of LH). CONCLUSION The primary and tertiary follicular stages maintain the best integrity and can be used after the vitrification of rat ovaries. doi.org/10.54680/fr23110110712.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.