低聚糖对链脲佐菌素诱导的糖尿病大鼠抗氧化、脂质及炎症的影响

K. Boyanov, Mariya Choneva, I. Dimov, I. Dimitrov, T. Vlaykova, F. Gerginska, S. Delchev, P. Hrischev, K. Georgieva, A. Bivolarska
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引用次数: 3

摘要

益生元,肠道微生物发酵物质,延缓I型糖尿病的发展。在本研究中,我们研究了两种益生元(低聚半乳糖和低聚木糖)对链脲佐菌素诱导的糖尿病大鼠的抗氧化保护、血脂水平和炎症活性的影响。研究了丙二醛、8-羟基-2′-脱氧鸟苷、血浆铁还原能力(FRAP)、甘油三酯、总胆固醇(TC)、高密度脂蛋白、c反应蛋白(CRP)和白细胞介素-6。用链脲佐菌素诱导雄性Wistar实验大鼠糖尿病,非糖尿病对照组注射生理盐水。然后给实验动物口服低聚糖。采用酶联免疫吸附法对砍头后采集的血液进行分析。修改后的方案仅用于测量FRAP值。低聚半乳糖和低聚木糖降低糖尿病大鼠丙二醛水平(p < 0.05)。低聚半乳糖降低了实验动物血清8-羟基-2′-脱氧鸟苷水平(p = 0.01),低聚木糖提高了FRAP水平(p < 0.05)。低聚糖均不影响三酰甘油和白细胞介素-6浓度,但半乳糖低聚糖降低了糖尿病动物的TC和CRP水平。两种低聚糖均对糖尿病大鼠具有抗氧化保护作用,但对其脂质和炎症谱的影响较小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of oligosaccharides on the antioxidant, lipid and inflammatory profiles of rats with streptozotocin-induced diabetes mellitus
Abstract Prebiotics, gut microbiota-fermentable substances, delay the development of type I diabetes. In the present study, we investigated the effect of two prebiotics (galacto-oligosaccharides and xylo-oligosaccharides) on the antioxidant protection, lipid profile, and inflammatory activity of rats with streptozotocin-induced diabetes. The following markers were studied – malondialdehyde, 8-hydroxy-2′-deoxyguanosine, ferric reducing ability of plasma (FRAP), triacylglycerols, total cholesterol (TC), high-density lipoproteins, C-reactive protein (CRP), and interleukin-6. Diabetes was induced in male Wistar experimental rats by streptozotocin injection, while the non-diabetic controls were injected with saline. Afterward the oligosaccharides were administered orally to the experimental animals. The blood collected following the decapitation was analyzed by ELISA. A modified protocol was used only for measuring the FRAP values. The galacto-oligosaccharides and xylo-oligosaccharides lowered the malondialdehyde levels in the diabetic rats (p < 0.05). The galacto-oligosaccharides decreased the serum levels of 8-hydroxy-2′-deoxyguanosine (p = 0.01), while the xylo-oligosaccharides increased the FRAP (p < 0.05) in the experimental animals. None of the oligosaccharides affected triacylglycerol and interleukin-6 concentrations, but the galacto-oligosaccharides decreased the TC and CRP levels in the diabetic animals. Both oligosaccharides exert a beneficial effect on the antioxidant protection of the diabetic rats, but have a minor effect on their lipid and inflammatory profiles.
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