植物乳杆菌 06CC2 能上调高脂饮食 Balb/c 小鼠肠道 ZO-1 蛋白和胆汁酸代谢。

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Bioscience of Microbiota, Food and Health Pub Date : 2024-01-01 Epub Date: 2023-07-31 DOI:10.12938/bmfh.2023-002
Masao Yamasaki, Yuko Miyamoto, Kenjirou Ogawa, Kazuo Nishiyama, Chuluunbat Tsend-Ayush, Yiran Li, Tatsuya Matsusaki, Tomoki Nakano, Masahiko Takeshita, Yuo Arima
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引用次数: 0

摘要

本研究评估了从蒙古奶制品中分离的植物乳杆菌06CC2(LP06CC2)对以高脂饮食(HFD)喂养的Balb/c小鼠的脂质代谢和肠道紧密连接相关蛋白的影响。给小鼠喂食高脂饮食八周后,对其血浆和肝脏脂质参数以及肠道紧密连接相关因子进行了评估。LP06CC2可轻微减少脂肪组织质量。此外,它还能剂量依赖性地降低血浆总胆固醇(TC)。高密度脂蛋白膳食往往会增加血浆内毒素水平并抑制肠道ZO-1的表达,而低剂量的LP06CC2则会增加ZO-1的表达并降低血浆脂多糖水平。此外,低剂量的 LP06CC2 有助于乳酸杆菌的适度积累,梭状芽孢杆菌簇 IV 显著减少,梭状芽孢杆菌簇 XVIII 增加。粪便和盲肠内容物中胆汁酸(BAs)的分析结果表明,LP06CC2 低剂量组的次级胆汁酸和共轭胆汁酸呈下降趋势。此外,高剂量的 LP06CC2 会导致乳酸杆菌的过度积累,并且不能增加肠道 ZO-1 和闭塞素的表达,而 LP06CC2 饲喂组小鼠粪便中丁酸盐的含量会随剂量增加而增加。最后,适当剂量的 LP06CC2 可保护肠道屏障功能不受高纤维食物的影响,并调节 BA 代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lactiplantibacillus plantarum 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet.

The effects of Lactiplantibacillus plantarum 06CC2 (LP06CC2), which was isolated from a Mongolian dairy product, on lipid metabolism and intestinal tight junction-related proteins in Balb/c mice fed a high-fat diet (HFD) were evaluated. The mice were fed the HFD for eight weeks, and the plasma and hepatic lipid parameters, as well as the intestinal tight junction-related factors, were evaluated. LP06CC2 slightly reduced the adipose tissue mass. Further, it dose-dependently decreased plasma total cholesterol (TC). The HFD tended to increase the plasma level of endotoxin and suppressed intestinal ZO-1 expression, whereas a low LP06CC2 dose increased ZO-1 expression and tended to reduce the plasma lipopolysaccharide level. Furthermore, a low LP06CC2 dose facilitated a moderate accumulation of Lactobacillales, a significant decrease in Clostridium cluster IV, and an increase in Clostridium cluster XVIII. The results obtained from analyzing the bile acids (BAs) in feces and cecum contents exhibited a decreasing trend for secondary and conjugated BAs in the low LP06CC2-dose group. Moreover, a high LP06CC2 dose caused excess accumulation of Lactobacillales and failed to increase intestinal ZO-1 and occludin expression, while the fecal butyrate level increased dose dependently in the LP06CC2-fed mice. Finally, an appropriate LP06CC2 dose protected the intestinal barrier function from the HFD and modulated BA metabolism.

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来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
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