{"title":"荆芥叶乙醇提取物体外稳定性及对人红细胞的聚集作用。-","authors":"O. B. Eze, O. Nwodo","doi":"10.5455/jeim.150816.or.156","DOIUrl":null,"url":null,"abstract":"Abstract Aim: This study aims to evaluate the membrane stability, Phospholipase A2 activity, prostaglandin synthase activity and aggregatory effect of ethanol leaves extract of Sida acuta Burm F. using an in-vitro heamolytic assay. Materials and methods: Two milliliters of blood collected from a healthy volunteer who has not taken drugs for last two weeks and ethanol extracts leaves of Sida acuta were used in the assay. Results: Apparently there was platelet aggregation at all but there was no minimal aggregation to denote in samples 1 and 2. There was a decrease in the supernatants of sample 1 of hypotonicity-induced haemolysis of human red blood cells incubated in normal saline but there was a high increase in the supernatants of sample 2 incubated with hypotonic solution. Inclusion of the extract concentrations 2, 4 and 8 mg/ml in the incubation medium (hypotonic solution) in samples 3, 4 and 5 decreased in a concentration dependent fashion. The absorption of haemolysed human red blood cells in isotonic solution showed the OD of 540nm:630nm to 2.15 for methaemoglobin while the OD of 540nm:570nm was 0.97 for deoxyhaemoglobin. In hypotonic solution where haemoglobin was released, the ratio of 540nm:630nm was 4.41 and 540nm:570nm was 1.20. The inclusion of the extract concentrations 2, 4 and 8 mg/ml in samples 3, 4 and 5 caused the ratio to decreased from1.69, 1.58 and 0.41 for methaemoglobin and 1.08, 0.86 and 0.41 for deoxyhaemoglobin. There was a decrease in activity of phospholipase A2 in samples 3, 4, 5 and 9 with the extract concentrations 2, 6, 12 and 16 mg/ml respectively but no decrease was observed in samples 6,7, 8 and 10, though no human red blood cells was included in samples 6, 7, 8, 9 and 10. There was a significant reduction (p 0.05) in prostaglandin synthase activity in sample 4 with extract concentration 25 mg/ml but a non-significant decrease (p 0.05) was observed in samples 3 and 5. Conclusion: The extract showed a significant inhibition of in-vitro haemolysis and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"26 1","pages":"134-138"},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"in-vitro stability and aggregatory effect of ethanol extract leaves of Sida acuta Burm F. on human erythrocyte. -\",\"authors\":\"O. B. Eze, O. Nwodo\",\"doi\":\"10.5455/jeim.150816.or.156\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Aim: This study aims to evaluate the membrane stability, Phospholipase A2 activity, prostaglandin synthase activity and aggregatory effect of ethanol leaves extract of Sida acuta Burm F. using an in-vitro heamolytic assay. Materials and methods: Two milliliters of blood collected from a healthy volunteer who has not taken drugs for last two weeks and ethanol extracts leaves of Sida acuta were used in the assay. Results: Apparently there was platelet aggregation at all but there was no minimal aggregation to denote in samples 1 and 2. There was a decrease in the supernatants of sample 1 of hypotonicity-induced haemolysis of human red blood cells incubated in normal saline but there was a high increase in the supernatants of sample 2 incubated with hypotonic solution. Inclusion of the extract concentrations 2, 4 and 8 mg/ml in the incubation medium (hypotonic solution) in samples 3, 4 and 5 decreased in a concentration dependent fashion. The absorption of haemolysed human red blood cells in isotonic solution showed the OD of 540nm:630nm to 2.15 for methaemoglobin while the OD of 540nm:570nm was 0.97 for deoxyhaemoglobin. In hypotonic solution where haemoglobin was released, the ratio of 540nm:630nm was 4.41 and 540nm:570nm was 1.20. The inclusion of the extract concentrations 2, 4 and 8 mg/ml in samples 3, 4 and 5 caused the ratio to decreased from1.69, 1.58 and 0.41 for methaemoglobin and 1.08, 0.86 and 0.41 for deoxyhaemoglobin. There was a decrease in activity of phospholipase A2 in samples 3, 4, 5 and 9 with the extract concentrations 2, 6, 12 and 16 mg/ml respectively but no decrease was observed in samples 6,7, 8 and 10, though no human red blood cells was included in samples 6, 7, 8, 9 and 10. There was a significant reduction (p 0.05) in prostaglandin synthase activity in sample 4 with extract concentration 25 mg/ml but a non-significant decrease (p 0.05) was observed in samples 3 and 5. Conclusion: The extract showed a significant inhibition of in-vitro haemolysis and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.\",\"PeriodicalId\":16091,\"journal\":{\"name\":\"Journal of Experimental and Integrative Medicine\",\"volume\":\"26 1\",\"pages\":\"134-138\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental and Integrative Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/jeim.150816.or.156\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/jeim.150816.or.156","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
in-vitro stability and aggregatory effect of ethanol extract leaves of Sida acuta Burm F. on human erythrocyte. -
Abstract Aim: This study aims to evaluate the membrane stability, Phospholipase A2 activity, prostaglandin synthase activity and aggregatory effect of ethanol leaves extract of Sida acuta Burm F. using an in-vitro heamolytic assay. Materials and methods: Two milliliters of blood collected from a healthy volunteer who has not taken drugs for last two weeks and ethanol extracts leaves of Sida acuta were used in the assay. Results: Apparently there was platelet aggregation at all but there was no minimal aggregation to denote in samples 1 and 2. There was a decrease in the supernatants of sample 1 of hypotonicity-induced haemolysis of human red blood cells incubated in normal saline but there was a high increase in the supernatants of sample 2 incubated with hypotonic solution. Inclusion of the extract concentrations 2, 4 and 8 mg/ml in the incubation medium (hypotonic solution) in samples 3, 4 and 5 decreased in a concentration dependent fashion. The absorption of haemolysed human red blood cells in isotonic solution showed the OD of 540nm:630nm to 2.15 for methaemoglobin while the OD of 540nm:570nm was 0.97 for deoxyhaemoglobin. In hypotonic solution where haemoglobin was released, the ratio of 540nm:630nm was 4.41 and 540nm:570nm was 1.20. The inclusion of the extract concentrations 2, 4 and 8 mg/ml in samples 3, 4 and 5 caused the ratio to decreased from1.69, 1.58 and 0.41 for methaemoglobin and 1.08, 0.86 and 0.41 for deoxyhaemoglobin. There was a decrease in activity of phospholipase A2 in samples 3, 4, 5 and 9 with the extract concentrations 2, 6, 12 and 16 mg/ml respectively but no decrease was observed in samples 6,7, 8 and 10, though no human red blood cells was included in samples 6, 7, 8, 9 and 10. There was a significant reduction (p 0.05) in prostaglandin synthase activity in sample 4 with extract concentration 25 mg/ml but a non-significant decrease (p 0.05) was observed in samples 3 and 5. Conclusion: The extract showed a significant inhibition of in-vitro haemolysis and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.