海洋细菌中链和长链烷烃羟化酶生产全细胞生物催化剂

A. Thontowi, E. Yetti, Yopi Yopi
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引用次数: 2

摘要

烷烃是原油的主要成分,可被烷烃羟化酶水解。烷烃羟化酶根据烷烃的链长分为短链长/SCL (C2-C4)、中链长/MCL (C5-C17)和长链长/LCL (C>18)三类。本研究的目的是表征和鉴定这些细菌中的烷烃降解菌。在MCL (Pentane-C5H12、Decane-C10H22和Pentadecane-C15H32)和LCL (n-Paraffin-C12H19C17和Pristane-C19H40分支)上培养30株海洋菌株。研究表明,29株菌株具有降解烷烃化合物的能力,14株菌株在MCL和LCL培养基上生长,11株菌株在MCL和n-LCL培养基上生长,3株菌株仅在MCL培养基上生长,1株菌株仅在n-LCL培养基上生长。生长试验结果表明,29株分离株具有中链烷烃单加氧酶和长链烷烃羟化酶。通过16S rDNA基因分析,共获得29种油降解菌,分别为a-变形菌属(57%)、g-变形菌属(30%)、Flavobacteria(7%)、Bacilli(3%)和Propionibacteriales(3%)。g-变形菌和a-变形菌似乎在烷烃生物降解中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria
Alkanes are  major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely a-proteobacteria (57 %), g-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). g-Proteobacteria and a-proteobacteria which seems to play an important role in the alkane biodegradation.
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