高度并行微流控平台中罕见表观遗传癌症生物标志物的数字DNA序列分析

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII) Pub Date : 2019-06-23 DOI:10.1109/TRANSDUCERS.2019.8808784

C. O’Keefe, Sixuan Li, Tza-Huei Wang

{"title":"高度并行微流控平台中罕见表观遗传癌症生物标志物的数字DNA序列分析","authors":"C. O’Keefe, Sixuan Li, Tza-Huei Wang","doi":"10.1109/TRANSDUCERS.2019.8808784","DOIUrl":null,"url":null,"abstract":"We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.","PeriodicalId":6672,"journal":{"name":"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)","volume":"27 1","pages":"2-5"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform\",\"authors\":\"C. O’Keefe, Sixuan Li, Tza-Huei Wang\",\"doi\":\"10.1109/TRANSDUCERS.2019.8808784\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.\",\"PeriodicalId\":6672,\"journal\":{\"name\":\"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)\",\"volume\":\"27 1\",\"pages\":\"2-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/TRANSDUCERS.2019.8808784\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/TRANSDUCERS.2019.8808784","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

我们提出了一个微流控平台，通过高度并行的数字熔化评估，对罕见肿瘤来源DNA的异质表观遗传模式进行分子-分子检测。我们介绍了一种多层几何策略，可以高效地捕获微阵列中的单分子。我们展示了对一种经典肿瘤抑制基因p14ARF罕见的异质性甲基化DNA的敏感检测和全面分析，甲基化/非甲基化的频率低至0.0001%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform

We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)

自引率

0.00%

发文量