Krung Hyun Sohn , Chan Yong Lee , Ke Ho Lee , Young Je Cho , Jae Heung Lee
{"title":"金链霉菌将5′-肌苷酸转化为肌苷","authors":"Krung Hyun Sohn , Chan Yong Lee , Ke Ho Lee , Young Je Cho , Jae Heung Lee","doi":"10.1016/0385-6380(88)90092-1","DOIUrl":null,"url":null,"abstract":"<div><p>The production of inosine by microbial conversion of 5′-inosinic acid (IMP) was investigated. Among the various strains of <em>Streptomyces</em> and <em>Bacillus</em> tested, <em>Streptomyces aureus</em> NCIB 9803 was selected for the microbial conversion process due to its high IMP-degrading activity. A maximum conversion yield of 0.43 (86% of the theoretical value) was obtained when IMP was added to the culture medium at 24 h. Kinetic studies with [8-<sup>14</sup>C] IMP showed that the difference from the theoretical values mainly attributable to the uptake of inosine by <em>S. aureus</em>.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 5","pages":"Pages 585-589"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90092-1","citationCount":"0","resultStr":"{\"title\":\"Conversion of 5′-inosinic acid to inosine by Streptomyces aureus\",\"authors\":\"Krung Hyun Sohn , Chan Yong Lee , Ke Ho Lee , Young Je Cho , Jae Heung Lee\",\"doi\":\"10.1016/0385-6380(88)90092-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The production of inosine by microbial conversion of 5′-inosinic acid (IMP) was investigated. Among the various strains of <em>Streptomyces</em> and <em>Bacillus</em> tested, <em>Streptomyces aureus</em> NCIB 9803 was selected for the microbial conversion process due to its high IMP-degrading activity. A maximum conversion yield of 0.43 (86% of the theoretical value) was obtained when IMP was added to the culture medium at 24 h. Kinetic studies with [8-<sup>14</sup>C] IMP showed that the difference from the theoretical values mainly attributable to the uptake of inosine by <em>S. aureus</em>.</p></div>\",\"PeriodicalId\":15702,\"journal\":{\"name\":\"Journal of Fermentation Technology\",\"volume\":\"66 5\",\"pages\":\"Pages 585-589\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0385-6380(88)90092-1\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0385638088900921\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0385638088900921","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Conversion of 5′-inosinic acid to inosine by Streptomyces aureus
The production of inosine by microbial conversion of 5′-inosinic acid (IMP) was investigated. Among the various strains of Streptomyces and Bacillus tested, Streptomyces aureus NCIB 9803 was selected for the microbial conversion process due to its high IMP-degrading activity. A maximum conversion yield of 0.43 (86% of the theoretical value) was obtained when IMP was added to the culture medium at 24 h. Kinetic studies with [8-14C] IMP showed that the difference from the theoretical values mainly attributable to the uptake of inosine by S. aureus.
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