巴格达市犬类分离沙门氏菌部分毒力、抗菌耐药基因的检测

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-06-25 DOI:10.36103/ijas.v54i3.1756

Maher. S. Al – Rubaye, Atheer. A. Al-doori

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引用次数: 0

摘要

在巴格达市训练警犬和军犬搜寻毒品、爆炸物的学院，对250只(250只)腹泻犬和非腹泻犬(雄性和雌性多品种犬)、当地犬和来自不同临床病例的宠物犬进行了测试。所有样本均在无菌直肠拭子中获得，并运送到微生物实验室进行沙门氏菌的培养和鉴定。该研究得出16例(6.4%)沙门氏菌阳性。毒力基因研究得到16个(6.4%)invA基因，invf13(5.2%)和c16(6.4%)。采用Bauer-Kirby法对16株(6.4%)分离菌株进行了抗生素敏感性试验，结果表明环丙沙星是临床最常用的抗沙门氏菌药物。聚合酶链反应(PCR)检测环丙沙星gyrA基因耐药基因，阳性分离株有16株(6.4%)。

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DETECTION OF SOME VIRULENCE, ANTIBACTERIAL RESISTANCE GENES FOR SALMONELLA ISOLATED FROM DOGS IN BAGHDAD CITY

Two hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which is the most clinical antibiotic drug of choice against Salmonella infection . Detection of gyrA gene resistance gene for ciprofloxacin using Polymerase chain reaction (PCR) gave 16 (6.4%) for all positive isolates.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.