Salam (Syzygium polyantha Wight)叶中黄酮类化合物的分离

A. Pasaribu
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引用次数: 0

摘要

采用甲醇溶剂浸渍法从沙兰叶中分离黄酮类化合物。得到的甲醇提取物蒸发后用乙酸乙酯溶解。然后将溶液浓缩并蒸发。乙酸乙酯馏分用甲醇溶解,用正己烷溶剂拆分。甲醇层蒸发后用本尼迪克特试剂测试,然后用6%的盐酸加热酸化。然后用氯仿提取分离。以硅胶为固定相,正己烷:乙酸乙酯分别以90:10、80:20、70:30、60:40 v/v为流动相,采用柱层析法分离氯仿层。纯化合物为膏状,棕红色,质量= 8.5 mg, Rf=0.60。与黄酮类化合物为正反应。通过紫外可见光谱、红外光谱和核磁共振光谱对化合物进行了进一步鉴定。光谱数据表明该化合物为类黄酮。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation of Flavonoid Compounds from Salam (Syzygium polyantha Wight) Leaves
Isolation of flavonoid compounds from Salam (Syzygium polyantha Wight) leaves has been done by maceration technique with methanol solvent. Methanol extract obtained was evaporated and dissolved with ethyl acetate. The solution was then concentrated and evaporated. The ethyl acetate fraction was dissolved with methanol and partitioned with n-hexane solvent. The methanol layer was evaporated and then tested with Benedict’s reagent and then acidified with HCl 6% while heated. It was then extracted partition with chloroform. The chloroform layer was separated using Column Chromatography with silica gel as the stationary phase and n-hexane: ethyl acetate 90:10, 80:20, 70:30, and 60:40 v/v, respectively as the mobile phase. The pure compound was paste, brownish red, mass = 8.5 mg, and Rf=0.60. It was a positive reaction with flavonoid compound reagents. The compound was further identified by using UV-Visible, FT-IR, and 1H-NMR spectroscopy. Spectroscopy data obtained indicated that the compound is a flavonoid.
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