Antimicrobial activity of Hydroxyapatite nanoparticles prepared from marble wastes

This work aims to prepare and characterize the hydroxyapatite (HAP) nanomaterials from marble wastes (the utilization of the building marble waste for reducing the environmental pollution hazards) and to study its capabilities as antimicrobial and antifungal agents of the prepared nanoparticle. The utilization of the marble waste as a source for calcium chloride and to be reacted with sodium hydrogen phosphate, for synthetization of hydroxyapatite nanoparticles, the prepared material is characterized, tested, and analyzed using X-ray diffraction (XRD), Scan Electron Microscope (SEM) with Energy Dispersive X-Ray analysis (EDAX) techniques. The antimicrobial activity of prepared hydroxyapatite nanomaterial is tested using a well diffusion method with different types of bacteria (Gram-negative): Escherichia. Coli, Salmonella paratyphi, Pseudomonas earuginosa, and Alcaligenes aquatilis and bacteria (Gram- positive): Staphylococcus aureus, and Streptococcacea pneumonia. The antifungal efficacy of HAP nanoparticles is tested for different species of Aspergillus niger, Aspergillus flavus, and Penicillium SP. The diameter of the inhibitory zone shows the sensitivity of the microorganism to HAP nanoparticles in a greater inhibition against Gram- positive Staphylococcus aureus and Streptococcacea pneumonia, at 100% DMSO concentration. The diameter of the inhibition zone was 03.70 mm, when compared with other types of bacteria. The diameter of the inhibitory zone showed the sensitivity of the microorganism to HAP nanoparticles in a greater inhibition against Penicillium SP at 100% DMSO concentration, the inhibition zone diameter was 2.20 mm, when compared with other Aspergillus niger and Aspergillus flavus fungal species. Based on obtained results for the HAP nanoparticles prepared from the marble wastes have antibacterial effects on both Gram-negative (E. coli) and Gram-positive (S. aureus) strains.