粘胶罗氏菌DY-18的全基因组序列:来自持续性根尖牙周炎病变的具有致密网状结构的临床分离物

K. Yamane, T. Nambu, T. Yamanaka, Chiho Mashimo, Chieko Sugimori, K. Leung, H. Fukushima
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引用次数: 23

摘要

粘胶罗氏菌是一种存在于人类口腔和咽部的机会致病菌。我们发现黏液胞杆菌DY-18是一种来自持续性根尖牙周炎病变的临床分离物,具有生物膜样结构。mucilaginosa ATCC25296也有类似的结构。为了进一步研究这些结构,我们确定了DY-18的全基因组序列,发现其染色体长度为2.26 mb。对于已知影响许多细菌生物膜形成的应激反应系统,DY-18基因组仅具有两个sigma因子基因。其中一个编码了一个额外的sigma因子,其启动子结合活性可能在响应环境刺激时受到调节。此外,在该基因组中还发现了几个分配给双组分信号转导系统的基因。据我们所知,这是mucilaginosa物种的第一个完整基因组,我们的数据提出了这种生物通过这些应激反应系统调节生物膜表型的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complete Genome Sequence of Rothia mucilaginosa DY-18: A Clinical Isolate with Dense Meshwork-Like Structures from a Persistent Apical Periodontitis Lesion
Rothia mucilaginosa is an opportunistic pathogen in the human oral cavity and pharynx. We found that R. mucilaginosa DY-18, a clinical isolate from a persistent apical periodontitis lesion, had biofilm-like structures. Similar structures were also observed on R. mucilaginosa ATCC25296. To further study these structures, we determined the complete genome sequence of DY-18 and found it a 2.26-Mb chromosome. Regarding stress responsive systems known to affect biofilm formation in many bacteria, DY-18 genome possessed only two sigma factor genes. One of these encoded an additional sigma factor whose promoter-binding activity may be regulated in response to environmental stimuli. Additionally, several genes assigned to two-component signal transduction systems were presented in this genome. To the best of our knowledge, this is the first complete genome of R. mucilaginosa species and our data raise the possibility that this organism regulates the biofilm phenotype through these stress responsive systems.
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