极低频脉冲磁场对脂质体悬液中H2O2-和Fe2+诱导的自由基脂质氧化的影响

IF 0.1 Q4 INTERNATIONAL RELATIONS
V. Martynyuk, Yuri V. Tseyslyer
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The magnetic field pulses were rectangular in shape with variable polarity for a period of oscillations. The induction of PMF was monitored using microteslameter G-79. The series of extremely low frequencies (5–80 Hz) and induction (5–500 μT) of PMF was chosen due to their environmental and physiological significance.\n\nResults: PMF of different frequencies with induction of 5 and 50 μT did not affect (p>0.05) H2O2- and Fe2+-induced lipid oxidation in liposomal suspensions. Statistically significant changes (p<0.05) were revealed only when liposomal suspensions were exposed to PMF with induction of 500 μT. It was found that the action of PMF with the frequency of 8 Hz 500 μT significantly inhibited H2O2-induced and enhanced Fe2+-induced chemiluminescence. This effect is associated with inhibition of the decomposition and with the accumulation of phospholipid hydroperoxides, which decompose and recombine in the presence of Fe2+ ions, which is accompanied by stronger chemiluminescence. 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引用次数: 0

摘要

背景:长期以来,实验生物学和医学对涉及活性氧的自由基过程给予了特别的关注。在电磁生物学中,由于发现了磁场对自由基过程的自旋机制,对生物膜中自由基氧化的兴趣大大增加。目前,这些机制被认为是生物体内磁接受过程的关键。脂质体作为生物膜最简单的模型,经常被用来研究不同因素对膜结构和功能性质的主要作用机制。然而,生态显著极低频磁场对脂质体混悬液中自由基氧化的影响尚未得到足够的研究。目的:阐明极低频脉冲磁场(ELF PMF)对脂质体混悬液中H2O2-和Fe2+诱导的天然磷脂过氧化作用的影响特点。材料与方法:采用pH=7.4的磷酸盐缓冲液脂质体悬液。根据文献和自己的光散射结果,脂质体的平均直径约为500Å。用单光子计数装置记录了脂质体悬浮液的超弱化学发光。它由放置测试样品的光绝缘试管单元,以及用于创建PMF的温度传感器和螺线管组成。测试样品与光电子倍增器的光学接触是通过光导进行的。记录系统由一个宽带光电倍增管探测器- FEU-130组成,温度为- 20℃。采用脉冲分析仪AI-256分离出与单个化学发光光量子配准相对应的有用信号。光电倍增管探测器上的电压施加在该探测器的电流-电压特性范围内,其中随机电压波动对有用信号的测量影响最小。在规定的时间间隔内记录的光量子数表征了实验样品中脂质自由基氧化过程的总体强度。脉冲磁场是使用位于试管部分的螺线管线圈产生的。PMF使用串行发生器G6-28创建。磁场脉冲呈矩形形状,在振荡周期内具有可变极性。用G-79微透镜监测PMF的诱导。选取极低频(5 ~ 80 Hz)和感应(5 ~ 500 μT)的PMF系列,主要考虑其环境和生理意义。结果:不同频率PMF在5 μT和50 μT诱导下对H2O2-和Fe2+诱导的脂质氧化无显著影响(p>0.05)。只有500 μT诱导PMF暴露于脂质体悬液时,才有统计学意义(p<0.05)。实验发现,频率为8 Hz 500 μT的PMF能显著抑制h2o2诱导的化学发光,增强Fe2+诱导的化学发光。这种效应与抑制分解和磷脂氢过氧化物的积累有关,磷脂氢过氧化物在Fe2+离子存在下分解和重组,并伴有较强的化学发光。化学发光强度的动力学对PMF频率的依赖性的研究表明,在高达30 Hz的范围内,存在一定的依赖性。然而,PMF对h2o2诱导氧化相的抑制作用并不总是伴随着Fe2+依赖性化学发光闪光幅度的统计学显著增加,这表明PMF在一定频率下具有普遍的抑制作用。结论:极低频PMF仅在诱导量超过几百微特斯拉时对脂质体悬液中自由基氧化有统计学意义。这表明PMF对所选膜模型条件下自由基过程的影响主要是通过决定自由基重组的自旋相互作用来实现的。诱导降低一个或两个数量级,以及PMF频率在50 Hz以上的增加,导致该物理因素对脂质体悬浮液中脂质自由基氧化强度影响的有效性降低。对ELF PMF影响最敏感的是h2o2诱导的脂质自由基氧化阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of impulse magnetic fields of extremely low frequencies on H2O2- and Fe2+-induced free radical lipid oxidation in liposomal suspensions
Background: For a long time, special attention in experimental biology and medicine is paid to free radical processes involving reactive oxygen species. In electromagnetic biology, the interest in free radical oxidation in biological membranes has increased significantly due to the discovery of spin mechanisms of magnetic fields on free radical processes. In the present day, these mechanisms are considered to be key in the processes of magnetoreception in living organisms. Liposomes, as the simplest models of biological membranes, are often used to study the primary mechanisms of action of different factors on the structural and functional properties of membranes. However, the influence of ecological significant extremely low-frequency magnetic fields on free radical oxidation in liposomal suspensions has not been studied enough. Objectives: The elucidation of the peculiarities of the influence of the extremely low frequency pulsed magnetic fields (ELF PMF) on H2O2- and Fe2+-induced peroxidation of natural phospholipids in liposomal suspensions. Materials and methods: The liposomal suspensions in phosphate buffer pH=7.4 were used. According to the literature and own results on light scattering the average diameter of liposomes was about 500Å. Ultra-weak chemiluminescence of liposomal suspensions was recorded using a device that operated in the mode of single photons counting. It consisted of a light-insulated cuvette unit where the test samples were placed, as well as a temperature sensor and a solenoid, which was used to create the PMF. Optical contact of the test samples with the photoelectron multiplier was carried out using a light guide. The recording system consisted of a broadband photomultiplier tube detector — FEU-130, which was at a temperature of –20°C. The pulse analyzer AI-256 was used to separate the useful signal that corresponded to the registration of single chemiluminescence light quanta. The voltage on the photomultiplier tube detector was applied in the range of current-voltage characteristics of this detector, in which a random voltage fluctuation had a minimal effect on the measurement of the useful signal. The number of light quanta that were recorded for defined time intervals characterized the overall intensity of the process of free radical oxidation of lipids in the experimental samples. The pulsed magnetic field was created using a solenoid coil located in the cuvette part. PMF was created using a serial generator G6-28. The magnetic field pulses were rectangular in shape with variable polarity for a period of oscillations. The induction of PMF was monitored using microteslameter G-79. The series of extremely low frequencies (5–80 Hz) and induction (5–500 μT) of PMF was chosen due to their environmental and physiological significance. Results: PMF of different frequencies with induction of 5 and 50 μT did not affect (p>0.05) H2O2- and Fe2+-induced lipid oxidation in liposomal suspensions. Statistically significant changes (p<0.05) were revealed only when liposomal suspensions were exposed to PMF with induction of 500 μT. It was found that the action of PMF with the frequency of 8 Hz 500 μT significantly inhibited H2O2-induced and enhanced Fe2+-induced chemiluminescence. This effect is associated with inhibition of the decomposition and with the accumulation of phospholipid hydroperoxides, which decompose and recombine in the presence of Fe2+ ions, which is accompanied by stronger chemiluminescence. The study of the dependence of the dynamics of the chemiluminescence intensity on the frequency of the PMF indicates the presence of a certain dependence of the effects in the range of up to 30 Hz. However, the inhibitory effect of PMF for the H2O2-induced oxidation phase is not always accompanied by a statistically significant increase in the amplitude of Fe2+-dependent light flash of chemiluminescence that indicates the general inhibitory effect of PMF at a certain frequency. Conclusions: PMF of extremely low frequencies statistically significantly affects the free radical oxidation in liposomal suspensions only at inductions exceeding several hundred microteslas. This indicates the effect of PMF on free radical processes for the conditions of the selected membrane model is realized mainly through spin interactions that determine the recombination of free radicals. The decrease of induction by one or two orders of magnitude, as well as increasing in frequency of the PMF above 50 Hz, leads to a decrease in the effectiveness of the influence of this physical factor on the intensity of lipid-free radical oxidation in liposomal suspensions. The most sensitive to the influence of ELF PMF is the phase of H2O2-induced free radical oxidation of lipids.
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来源期刊
Meridiano 47-Journal of Global Studies
Meridiano 47-Journal of Global Studies INTERNATIONAL RELATIONS-
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