Li-Mei Zhang, Eleonora Silvano, Branko Rihtman, Maria Aguilo-Ferretjans, Bing Han, Wei Shi, Yin Chen
{"title":"重氮营养细菌水痘克雷伯菌W12限磷破坏固氮的生化机制","authors":"Li-Mei Zhang, Eleonora Silvano, Branko Rihtman, Maria Aguilo-Ferretjans, Bing Han, Wei Shi, Yin Chen","doi":"10.1002/sae2.12024","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Biological nitrogen (N) fixation (BNF) plays a key role in nitrogen supply in agricultural and natural ecosystems. Harnessing BNF can substantially reduce dependence on chemical fertilizer in agroecosystems and hence can contribute to sustainable agriculture. However, a number of field studies have demonstrated that BNF can be largely suppressed in phosphorus (P)-deficient environments, while the underlying mechanism is not well understood.</p>\n </section>\n \n <section>\n \n <h3> Materials & Methods</h3>\n \n <p>In this study, comparative proteomics and lipidomics analyses were conducted on a diazotrophic bacterium <i>Klebsiella variicola</i> W12 under P-deficient and P-replete conditions to gain insight into how P availability affects N fixation.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Under P deficiency, N fixation activity of <i>K. variicola</i> W12 was severely repressed. In response to P limitation, the bacterium synthesized P-free ornithine lipids to replace glycerophospholipids in its membrane to reduce cellular demand for P. Comparative proteomics showed that P limitation resulted in upregulation of the PhoBR two-component system, a range of organic and inorganic P uptake and transport systems, while nitrogenase and N-fixation-related transcriptional regulators NifL and NifA were downregulated.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>These results revealed lipid renovation as an adaptation strategy for N<sub>2</sub>-fixing microbes to survive under P stress and provided biochemical evidence on how P availability regulates BNF. A conceptual model of N–P coupling at the microbial metabolism level is therefore proposed. Our study provides a simple yet plausible explanation of how P deficiency suppresses BNF observed in the field and highlights the importance of regulating P availability to maximize the potential of BNF in agroecosystems for agriculture sustainable production.</p>\n </section>\n </div>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12024","citationCount":"2","resultStr":"{\"title\":\"Biochemical mechanism of phosphorus limitation impairing nitrogen fixation in diazotrophic bacterium Klebsiella variicola W12\",\"authors\":\"Li-Mei Zhang, Eleonora Silvano, Branko Rihtman, Maria Aguilo-Ferretjans, Bing Han, Wei Shi, Yin Chen\",\"doi\":\"10.1002/sae2.12024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>Biological nitrogen (N) fixation (BNF) plays a key role in nitrogen supply in agricultural and natural ecosystems. Harnessing BNF can substantially reduce dependence on chemical fertilizer in agroecosystems and hence can contribute to sustainable agriculture. However, a number of field studies have demonstrated that BNF can be largely suppressed in phosphorus (P)-deficient environments, while the underlying mechanism is not well understood.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials & Methods</h3>\\n \\n <p>In this study, comparative proteomics and lipidomics analyses were conducted on a diazotrophic bacterium <i>Klebsiella variicola</i> W12 under P-deficient and P-replete conditions to gain insight into how P availability affects N fixation.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Under P deficiency, N fixation activity of <i>K. variicola</i> W12 was severely repressed. In response to P limitation, the bacterium synthesized P-free ornithine lipids to replace glycerophospholipids in its membrane to reduce cellular demand for P. Comparative proteomics showed that P limitation resulted in upregulation of the PhoBR two-component system, a range of organic and inorganic P uptake and transport systems, while nitrogenase and N-fixation-related transcriptional regulators NifL and NifA were downregulated.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>These results revealed lipid renovation as an adaptation strategy for N<sub>2</sub>-fixing microbes to survive under P stress and provided biochemical evidence on how P availability regulates BNF. A conceptual model of N–P coupling at the microbial metabolism level is therefore proposed. Our study provides a simple yet plausible explanation of how P deficiency suppresses BNF observed in the field and highlights the importance of regulating P availability to maximize the potential of BNF in agroecosystems for agriculture sustainable production.</p>\\n </section>\\n </div>\",\"PeriodicalId\":100834,\"journal\":{\"name\":\"Journal of Sustainable Agriculture and Environment\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12024\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Sustainable Agriculture and Environment\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/sae2.12024\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Sustainable Agriculture and Environment","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/sae2.12024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Biochemical mechanism of phosphorus limitation impairing nitrogen fixation in diazotrophic bacterium Klebsiella variicola W12
Introduction
Biological nitrogen (N) fixation (BNF) plays a key role in nitrogen supply in agricultural and natural ecosystems. Harnessing BNF can substantially reduce dependence on chemical fertilizer in agroecosystems and hence can contribute to sustainable agriculture. However, a number of field studies have demonstrated that BNF can be largely suppressed in phosphorus (P)-deficient environments, while the underlying mechanism is not well understood.
Materials & Methods
In this study, comparative proteomics and lipidomics analyses were conducted on a diazotrophic bacterium Klebsiella variicola W12 under P-deficient and P-replete conditions to gain insight into how P availability affects N fixation.
Results
Under P deficiency, N fixation activity of K. variicola W12 was severely repressed. In response to P limitation, the bacterium synthesized P-free ornithine lipids to replace glycerophospholipids in its membrane to reduce cellular demand for P. Comparative proteomics showed that P limitation resulted in upregulation of the PhoBR two-component system, a range of organic and inorganic P uptake and transport systems, while nitrogenase and N-fixation-related transcriptional regulators NifL and NifA were downregulated.
Conclusion
These results revealed lipid renovation as an adaptation strategy for N2-fixing microbes to survive under P stress and provided biochemical evidence on how P availability regulates BNF. A conceptual model of N–P coupling at the microbial metabolism level is therefore proposed. Our study provides a simple yet plausible explanation of how P deficiency suppresses BNF observed in the field and highlights the importance of regulating P availability to maximize the potential of BNF in agroecosystems for agriculture sustainable production.