正己烷和乙酸乙酯提取物对M1极性激活的HMC3小胶质细胞MHCII表达降低的影响叶子

Burhan Ma’arif, Faisal Akhmal Muslikh, Abdul Malik Guhir, H. Fitri, Luqman Alfani Najib, Novyananda Salmasfattah, H. Laswati, M. Agil
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引用次数: 0

摘要

神经炎症是一种神经细胞逐渐丧失功能的病理状态,其中之一是由于雌激素缺乏。植物雌激素是一种结构和功能与17β-雌二醇相似的化合物,17β-雌二醇是雌激素最有效的天然形式。Semanggi (Marsilea crenatata Presl.)含有植物雌激素,可以替代17β-雌二醇维持器官稳态的功能,因此具有开发抗神经炎症药物的潜力。本研究旨在通过测定三叶正己烷和乙酸乙酯组分对HMC3小胶质细胞的主要组织相容性复合体II (MHC II)的表达,证明三叶正己烷和乙酸乙酯组分对HMC3小胶质细胞的抗神经炎作用。125;干扰素γ (IFN-γ)诱导HMC3小胶质细胞24小时后,给予250 μg/ml。采用免疫细胞化学(ICC)法和共聚焦激光扫描显微镜(CLSM)仪检测MHCⅱ的表达。结果表明,正己烷部位在250 μg/ml浓度下可降低MHC II的表达(p<0.05),且呈非单调剂量反应(NMDR)模式;乙酸乙酯部位在所有浓度下均可降低MHC II的表达(p<0.05)。相关试验结果表明,三叶乙酸乙酯部位对HMC3小胶质细胞的抗炎活性最好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Reduction of MHCII Expression on M1 Polarity Activated HMC3 Microglia Cells By n-Hexane And Ethyl Acetate Fraction of Semanggi (Marsilea crenata Presl.) Leaves
Neuroinflammatory is a pathological condition in which neuronal cells experience progressive loss of function, one of which is due to estrogen deficiency. Phytoestrogens are compound that has structure and function similar to 17β-estradiol, the most potent natural form of estrogen. Semanggi (Marsilea crenata Presl.) is known to contain phytoestrogens which can replace the function of 17β-estradiol in maintaining organ homeostasis, so that is potential to be developed as an anti-neuroinflammatory drug. This study was aimed to prove the anti-neuroinflammatory effect of the n-hexane and ethyl acetate fraction of Semanggi leaves on HMC3 microglia cells, by measuring the expression of major histocompatibility complex II (MHC II). Both fractions with a concentration of 62.5; 125; and 250 μg/ml were administered to HMC3 microglia cells which had been induced by interferon gamma (IFN-γ) for 24 hours. Measurement of MHC II expression were using immunocytochemistry (ICC) methods and confocal laser scanning microscopy (CLSM) instruments. The results showed that n-hexane fraction could reduce MHC II expression at concentrations of 250 μg/ml with p<0.05, and showed a non-monotonic dose response (NMDR) pattern, while ethyl acetate fraction could reduce MHC II expression at all concentrations with p<0.05. Based on the results of the correlation test, the best anti-neuroinflammatory activity on HMC3 microglia cells is showed by the ethyl acetate fraction of Semanggi leaves.
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