咖啡酸与低功率激光共照射对铜绿假单胞菌生存能力的影响

N. Gheibi, N. Khosroshahi, Marzieh Habibi
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引用次数: 1

摘要

背景:铜绿假单胞菌对抗生素的耐药性是一个很大的问题,特别是在烧伤和创面感染中。激光照射通过使微生物的蛋白质变性来影响微生物,这涉及到蛋白质的化学或物理性质的变化。目的:研究咖啡酸与低功率激光共照射对烧伤创面铜绿假单胞菌的影响。材料与方法:从德黑兰Shahid Motahhari医疗中心烧伤创面感染患者中采集10份细菌样本。本研究使用的He-Ne激光器输出功率为2 mW。结果:数据显著表明,与对照培养相比,咖啡酸和激光单独治疗都减少了菌落形成单位的数量。咖啡酸和激光在三个时间点共暴露菌悬液,菌落形成单位数分别为240.23±60.15、148.13±52.66和84.57±35。咖啡酸的最佳浓度为1.5和1.75 mM,当咖啡酸浓度为1.5 mM时,菌落数显著减少至280.78±59个(P = 0.008),而当咖啡酸浓度为1.75 mM时,菌落数显著减少至234.07±72.28个(P = 0.0001)。结论:咖啡酸处理可减少细菌生长,导致菌落形成数量减少。与对照、咖啡酸和单独使用激光相比,咖啡酸和激光在三个时间过程中同时作用在减少菌落形成方面显示出协同效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Caffeic Acid and Low-Power Laser Light Co-Exposure on Viability of Pseudomonas aeruginosa
Background: The resistance of Pseudomonas aeruginosa to antibiotics is a big problem, especially in burns and wound infections. Laser irradiation affects microorganisms by denaturing their proteins, which involves changes in the chemical or physical properties of the protein. Objectives: The aim of this study was to investigate the effect of caffeic acid and low-power laser light co-exposure on Pseudomonas aeruginosa isolated from burn wounds. Materials and Methods: Ten bacterial samples were collected from patients with burn wound infections at Shahid Motahhari medical center of Tehran. The He-Ne laser was used in this study with output power of 2 mW. Results: The data significantly indicated that both the caffeic acid and laser treatment alone reduced the number of colony-forming units compared to control cultures. Co-exposure of bacterial suspensions to caffeic acid and laser at three time points showed the following number of colony-forming units 240.23 ± 60.15, 148.13 ± 52.66 and 84.57 ± 35, respectively. The best concentrations of caffeic acid to achieve countable colonies were 1.5 and 1.75 mM. At the concentration of 1.5 mM of caffeic acid, the number of colonies significantly reduced to 280.78 ± 59 (P = 0.008) while at 1.75 mM the number of colonies reduced to 234.07 ± 72.28 (P = 0.0001). Conclusions: Caffeic acid treatment reduced bacterial growth and resulted in a decreased number of colony formation. The simultaneous effect of caffeic acid and laser at three time courses showed a synergic effect in reducing colony formation compared to the control and caffeic acid, and laser alone.
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