{"title":"杂化脂质体对NP2胶质瘤细胞生长的抑制作用","authors":"Keiji Kuwabara, H. Ichihara, Y. Matsumoto","doi":"10.35248/2157-2518.20.11.344","DOIUrl":null,"url":null,"abstract":"The inhibitory activity of hybrid liposomes (HLs), composed of 90 mol% L-α-dimyristoyl-phosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (25) dodecyl ether (C12(EO)25), was investigated in human glioma NP2 cells. HLs with a hydrodynamic diameter below 100 nm persisted for 4 weeks. The inhibitory effect of HLs on the proliferation of NP2 cells was evaluated. Induction of apoptosis in NP2 cells treated with HL was measured through a PI assay and the TUNEL method. HLs caused apoptosis in NP2 cells through the mitochondrial pathway. An increase of AIF protein expression was observed in HL-treated cells. Cellular membrane fluidity of NP2 cells was also increased as revealed by the fluorescence depolarization method. Enhanced HL accumulation in the membrane of NP2 cells was observed.","PeriodicalId":15209,"journal":{"name":"Journal of carcinogenesis & mutagenesis","volume":"15 1","pages":"1-5"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibitory Effect of Hybrid Liposomes on the Growth of NP2 Glioma Cells\",\"authors\":\"Keiji Kuwabara, H. Ichihara, Y. Matsumoto\",\"doi\":\"10.35248/2157-2518.20.11.344\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The inhibitory activity of hybrid liposomes (HLs), composed of 90 mol% L-α-dimyristoyl-phosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (25) dodecyl ether (C12(EO)25), was investigated in human glioma NP2 cells. HLs with a hydrodynamic diameter below 100 nm persisted for 4 weeks. The inhibitory effect of HLs on the proliferation of NP2 cells was evaluated. Induction of apoptosis in NP2 cells treated with HL was measured through a PI assay and the TUNEL method. HLs caused apoptosis in NP2 cells through the mitochondrial pathway. An increase of AIF protein expression was observed in HL-treated cells. Cellular membrane fluidity of NP2 cells was also increased as revealed by the fluorescence depolarization method. Enhanced HL accumulation in the membrane of NP2 cells was observed.\",\"PeriodicalId\":15209,\"journal\":{\"name\":\"Journal of carcinogenesis & mutagenesis\",\"volume\":\"15 1\",\"pages\":\"1-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of carcinogenesis & mutagenesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35248/2157-2518.20.11.344\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of carcinogenesis & mutagenesis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35248/2157-2518.20.11.344","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Inhibitory Effect of Hybrid Liposomes on the Growth of NP2 Glioma Cells
The inhibitory activity of hybrid liposomes (HLs), composed of 90 mol% L-α-dimyristoyl-phosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (25) dodecyl ether (C12(EO)25), was investigated in human glioma NP2 cells. HLs with a hydrodynamic diameter below 100 nm persisted for 4 weeks. The inhibitory effect of HLs on the proliferation of NP2 cells was evaluated. Induction of apoptosis in NP2 cells treated with HL was measured through a PI assay and the TUNEL method. HLs caused apoptosis in NP2 cells through the mitochondrial pathway. An increase of AIF protein expression was observed in HL-treated cells. Cellular membrane fluidity of NP2 cells was also increased as revealed by the fluorescence depolarization method. Enhanced HL accumulation in the membrane of NP2 cells was observed.
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