植物类黄酮槲皮素通过凋亡机制抑制表皮生长因子诱导的前列腺癌(PC3)细胞的存活和增殖

Firdous Ahmad Bhat, G. Sharmila, S. Balakrishnan, P. Raja Singh, N. Srinivasan, J. Arunakaran
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引用次数: 12

摘要

表皮生长因子(EGF)通过促进上皮恶性肿瘤细胞的增殖、存活、侵袭和转移发挥关键作用。肿瘤表皮生长因子受体(EGFR)的异常表达通常赋予更具侵袭性的表型,并且通常预示着不良预后。槲皮素是一种广泛存在于水果和蔬菜中的抗氧化类黄酮,作为潜在的抗癌物质受到广泛关注。前列腺癌是男性癌症相关死亡的最常见原因。在本研究中,我们研究了槲皮素对EGF诱导的PC-3细胞增殖、存活和凋亡信号分子的影响。槲皮素抑制egf刺激的EGFR、Akt、pi3k、PDK1和ERK1/2蛋白水平。比较槲皮素与pi3k抑制剂(LY294002)和MAPK抑制剂(PD98059)对EGF诱导的信号传导的抑制作用。槲皮素下调EGF诱导的Bcl-2表达,上调Bax蛋白水平。槲皮素处理显著提高了Caspase-3活性。吖啶橙和溴化乙啶染色表明,即使在EGF存在的情况下,槲皮素也能诱导细胞凋亡。综上所述,槲皮素抑制EGF诱导PC-3细胞存活、增殖并诱导细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidermal growth factor-induced prostate cancer (PC3) cell survival and proliferation is inhibited by quercetin, a plant flavonoid through apoptotic machinery

Epidermal growth factor (EGF) plays a key role in epithelial malignancies by enhancing cancer cell proliferation, survival, invasion, and metastasis. The aberrant expression of epidermal growth factor receptor (EGFR) by tumors typically confers a more aggressive phenotype and is often predictive of poor prognosis. Quercetin is an anti-oxidative flavonoid widely distributed in fruits and vegetables and have attracted much attention as potential anti-carcinogens. Prostate cancer is the most common cause of cancer related deaths in men. In the present study, we examined the effects of quercetin on EGF induced signaling molecules involved in proliferation, survival and apoptosis in PC-3 cells. EGF-stimulated EGFR, Akt, PI3 K, PDK1 and ERK1/2 protein levels were inhibited by quercetin. The inhibitory effects of quercetin on EGF induced signaling were compared with PI3 K inhibitor (LY294002) and MAPK inhibitor (PD98059). Quercetin down-regulated EGF induced Bcl-2 expression and upregulated Bax protein levels. Caspase-3 activity was significantly increased by quercetin treatment. Acridine orange and ethidium bromide staining showed that quercetin was able to induce apoptosis even in the presence of EGF. To conclude, the present study showed that quercetin inhibits EGF induced cell survival, proliferation and induced apoptosis in PC-3 cells.

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