Satoshi Suzuki, T. Hidaka, S. Sano, Gen Yoshida, F. Nishimura
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引用次数: 1
摘要
污水污泥与各类有机废弃物厌氧共消化产生的消化液可直接用作肥料。研究了利用消化液培养光合细菌以提高肥料品质的方法。以污水污泥种子为原料,用人工培养基富集8个月后,观察到Rhodospirillaceae的积累,已知为光合细菌,经测序检测,其检出率为总reads数的55%。将过滤后的污水污泥厌氧消化的消化液在光照下连续培养,初始只进行富集培养的播种。培养液中pufM基因拷贝数较高,这是光合细菌的一个指标。盆栽试验结果表明,小松(Brassica rapa var. perviridis)鲜重、叶绿素和类胡萝卜素含量显著高于对照(t检验,p < 0.05)。这些结果证实了在光照下培养过滤后的消化液能提高肥料品质。另一方面,在光照下对污泥进行厌氧消化培养消化液,初期只播种富集培养,盆栽试验结果差异不显著。说明在光照条件下培养过滤后的消化液是可行的。
Cultivation of Filtered Anaerobically Digested Sewage Sludge under Light Illumination for Improvement of Fertilizer Quality
The anaerobic co-digestion of sewage sludge and various types of organic waste produces digestate, which can be used directly as fertilizer. The cultivation of photosynthetic bacteria with digestate is focused on to improve the fertilizer quality. After eight months of enrichment with artificial culture media from a seed of sewage sludge, the accumulation of Rhodospirillaceae, which are known to be photosynthetic bacteria, was observed, and the detection ratio to the total number of reads was 55% by sequencing. Filtered digestate liquor from the anaerobic digestion of sewage sludge was continuously cultivated under light illumination, with the seeding of the enrichment culture only at the beginning. The cultivated liquor had a higher pufM gene copy number, which is an indicator of photosynthetic bacteria. Pot experiments showed that grown komatsuna ( Brassica rapa var. perviridis ) had significantly higher ( t-test, p < 0.05 ) fresh weight and ratios of chlorophyll and carotenoid. These results confirmed that the cultivation of filtered digestate liquor under light illumination improves the fertilizer quality. On the other hand, the cultivation of digestate by the anaerobic digestion of sewage sludge under light illumination with the seeding of the enrichment culture only at the beginning resulted in no significant difference in pot experiments. This showed that the cultivation of filtered digestate liquor under light illumination is practical.