幼年、亚成体和成体罗素蝰蛇(Daboia siamensis)毒液标本的成分和功能比较:与实验兔肾脏病理生理的相关性

IF 1.8 3区 医学 Q4 TOXICOLOGY
N. Chaiyabutr, L. Chanhome, T. Vasaruchapong, Panithi Laoungbua, O. Khow, A. Rungsipipat, O. Reamtong, V. Sitprija
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引用次数: 1

摘要

背景:东罗素毒蛇(Daboia siamensis)是导致急性肾衰竭的最重要的医学毒蛇之一。然而,幼鼠和成虫叮咬后的临床表现和肾脏病理生理变化尚未阐明。方法:本研究分析了暹罗丁在幼虫、亚成虫和成虫不同成熟阶段的毒液谱。通过比较幼年、亚成体和成体siamensis毒液标本的组成和功能特征,并将其与实验兔肾脏病理生理相关联,对各组相同的毒液进行酶、电泳和蛋白质组学分析,包括亚致死毒性(0.1 mg/kg iv.)检测身体功能。结果:比较研究发现,幼鱼毒液中磷脂酶A2、金属蛋白酶和丝氨酸蛋白酶含量较高,而亚成鱼和成鱼毒液中l -氨基酸氧化酶、磷酸二酯酶、kunitz型丝氨酸蛋白酶抑制剂、分解素家族和内皮生长因子含量较高。一项体内研究显示,成虫和亚成虫毒液引起持续的低血压和心动过缓,而血小板减少症是幼虫毒液的更典型的效果。所有年龄段的人都表现出肾脏血流动力学和电解质排泄的显著减少。幼蛇毒液引起的肾小管病变评分高于成虫和亚成虫毒液。结论:siamensis毒液的成分和活性在个体发生上发生了变化。中毒后肾功能的改变取决于不同毒液成分的协同作用或不同年龄毒液组中酶和非酶蛋白浓度的不成比例表达。幼鱼毒液中高比例的酶毒素蛋白质导致更大的肾毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper ( Daboia siamensis ): correlation with renal pathophysiology in experimental rabbits
Abstract Background: Eastern Russell’s viper (Daboia siamensis) is one of the most medically significant snakes responsible for the development of acute renal failure. However, variation of the clinical picture and renal pathophysiology following bites by young and adult D. siamensis have not been elucidated. Methods: In this study, we analyzed the venomic profiles of D. siamensis at different maturation stages of juvenile, subadult and adult groups. The same pooled venom from each group was subjected to enzymatic, electrophoretic and proteomic analysis, including sublethal toxicity (0.1 mg/kg iv.) examined on bodily functions by comparing the venom compositional and functional profiles among venom specimens from juvenile, subadult and adult D. siamensis by correlating them with the renal pathophysiology in experimental rabbits. Results: The comparative studies revealed that juvenile venom possessed higher phospholipase A2, metalloproteinase and serine proteinase levels, while subadult and adult venoms contained more L-amino acid oxidase, phosphodiesterase, the Kunitz-type serine protease inhibitor, disintegrin families and endothelial growth factor. An in vivo study revealed that the adult and subadult venoms caused persistent hypotension and bradycardia, while thrombocytopenia was a more characteristic effect of juvenile venom. All venom age groups showed significant reductions in renal hemodynamics and electrolyte excretions. The juvenile venom caused a higher tubulonephrosis lesion score than adult and subadult venoms. Conclusions: The D. siamensis venom shows an ontogenetic shift in its compositions and activities. Renal function alterations after envenomation depend on either the synergistic actions of different venom components or the disproportionate expression between the concentrations of enzymatic and non-enzymatic proteins in each age venom group. The high proportion of enzymatic toxin proteins in the juvenile venom results in greater nephrotoxicity.
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来源期刊
CiteScore
4.80
自引率
8.30%
发文量
39
审稿时长
6-12 weeks
期刊介绍: Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.
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