利用光诱导电泳和微流体技术快速富集细胞外囊泡

2020 IEEE 15th International Conference on Nano/Micro Engineered and Molecular System (NEMS) Pub Date : 2020-09-27 DOI:10.1109/NEMS50311.2020.9265578

Yi-Sin Chen, C. Lai, Chen-Wei Fan, Chihchen Chen, Gwo-Bin Lee

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引用次数: 0

摘要

本研究展示了一种利用集成微流体系统和光诱导电介质电泳(ODEP)技术富集和回收细胞外囊泡(ev)的新方法。这是首次通过ODEP在最小干预下实现无标签和无接触的富集和回收电动汽车。通过ODEP控制电动汽车的最佳驱动频率为20 kHz。采用集成式微流控光诱导EV分选芯片和自制控制系统，可在3 min内富集和回收20 μL EV样品，回收率为27.8%，具有广阔的EV相关应用前景。

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Rapid Enrichment of Extracellular Vesicles via Optically-induced Dielectrophoresis and Microfluidics

This study demonstrates a novel method to enrich and recover extracellular vesicles (EVs) by using an integrated microfluidic system and optically-induced dielectrophoresis (ODEP) technique. It is the first time that label-free and contact-free enrichment and recovery of EVs via ODEP with minimal intervention has been achieved. The optimal driving frequency (20 kHz) was found for EV manipulation via ODEP. The rapid and automatic procedure could be performed to enrich and recover 20 μL of the EV sample with a recovery rate of 27.8% within 3 min by using an integrated microfluidic optically-induced EV sorting chip and a home-made control system, showing great potential for further EV-associated applications.

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来源期刊

2020 IEEE 15th International Conference on Nano/Micro Engineered and Molecular System (NEMS)

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