R. Shagufta, B. Fomda, B. Gulnaz, Samad Lubna, Abiroo Jan, S. Mohd, Ahmad Junaid
{"title":"克什米尔三级保健机构不同表型方法测定革兰氏阴性杆菌AmpC β -内酰胺酶的流行情况","authors":"R. Shagufta, B. Fomda, B. Gulnaz, Samad Lubna, Abiroo Jan, S. Mohd, Ahmad Junaid","doi":"10.9734/bjmmr/2017/30462","DOIUrl":null,"url":null,"abstract":"RS designed the study BAF managed the literature searches and analyses of the study performed. the experimental process. SL, A. Jan and A. Junaid in analyses of the results. SM in analysis of results and the first of the ABSTRACT Context: AmpC type cephalosporinases are Ambler class C β -lactamases. They hydrolyze penicillins, cephalosporins; except the fourth-generation compounds and monobactams. To the best of our knowledge prevalence of AmpC in our hospital is not known. Aims: With this background the study was undertaken to detect the prevalence of AmpC lactamases in gram-negative bacilli at our institute. We also performed four different tests, i.e. AmpC disc test, modified Hodge test, modified three dimensional spot inoculation test on a single agar plate (three in one) and inhibitor based test using boronic acid for detecting AmpC production. Methods and Materials: Screening for AmpC was done by using cefoxitin discs. Organisms resistant to cefoxitin were tested for presence of AmpC by AmpC disc test, Modified Hodge test, Three dimensional spot inoculation test and Boronic acid disc test. Statistical Analysis: Statistical analysis was done by Chi Square test and Fishers Exact Test with the SPSS statistical programme (version 20.0). Results: A total of 1323 non duplicate Gram negative bacilli were isolated during a period of one year in the Department of microbiology SKIMS. Out of these 1323 isolates, 150 isolates were found to be resistant to cefoxitin and one or more third generation cephalosporin antibiotics or showed antagonism between cefoxitin and cefotaxim discs thus fulfilling the criteria and were included in the present study. Forty five (3.4%) were positive for AmpC beta lactamases. Boronic acid test detected 38 (25.3%), AmpC disc test 35 (23.3%), MHT 29 (19.3%) and MTDST detected 27 (18%). Conclusions: Inhibitor based test using boronic acid detected the maximum number of AmpC producing organisms. It is a simple and cost effective test that can be incorporated in routine susceptibility testing plate thus saving time also.","PeriodicalId":9249,"journal":{"name":"British journal of medicine and medical research","volume":"44 1","pages":"1-9"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Prevalence of AmpC Beta-lactamase in Gram Negative Bacilli by Different Phenotypic Methods in a Tertiary Care Institute in Kashmir\",\"authors\":\"R. Shagufta, B. Fomda, B. Gulnaz, Samad Lubna, Abiroo Jan, S. Mohd, Ahmad Junaid\",\"doi\":\"10.9734/bjmmr/2017/30462\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"RS designed the study BAF managed the literature searches and analyses of the study performed. the experimental process. SL, A. Jan and A. Junaid in analyses of the results. SM in analysis of results and the first of the ABSTRACT Context: AmpC type cephalosporinases are Ambler class C β -lactamases. They hydrolyze penicillins, cephalosporins; except the fourth-generation compounds and monobactams. To the best of our knowledge prevalence of AmpC in our hospital is not known. Aims: With this background the study was undertaken to detect the prevalence of AmpC lactamases in gram-negative bacilli at our institute. We also performed four different tests, i.e. AmpC disc test, modified Hodge test, modified three dimensional spot inoculation test on a single agar plate (three in one) and inhibitor based test using boronic acid for detecting AmpC production. Methods and Materials: Screening for AmpC was done by using cefoxitin discs. Organisms resistant to cefoxitin were tested for presence of AmpC by AmpC disc test, Modified Hodge test, Three dimensional spot inoculation test and Boronic acid disc test. Statistical Analysis: Statistical analysis was done by Chi Square test and Fishers Exact Test with the SPSS statistical programme (version 20.0). Results: A total of 1323 non duplicate Gram negative bacilli were isolated during a period of one year in the Department of microbiology SKIMS. Out of these 1323 isolates, 150 isolates were found to be resistant to cefoxitin and one or more third generation cephalosporin antibiotics or showed antagonism between cefoxitin and cefotaxim discs thus fulfilling the criteria and were included in the present study. Forty five (3.4%) were positive for AmpC beta lactamases. Boronic acid test detected 38 (25.3%), AmpC disc test 35 (23.3%), MHT 29 (19.3%) and MTDST detected 27 (18%). Conclusions: Inhibitor based test using boronic acid detected the maximum number of AmpC producing organisms. 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引用次数: 6
摘要
RS设计了研究,BAF管理了研究的文献检索和分析。实验过程。SL, A. Jan和A. Junaid对结果进行了分析。摘要背景:AmpC型头孢菌素酶是Ambler类C β -内酰胺酶。它们水解青霉素类,头孢菌素类;除了第四代化合物和单巴克坦。据我们所知,本院AmpC患病率尚不清楚。目的:在此背景下,对我院革兰氏阴性杆菌AmpC内酰胺酶的流行情况进行检测。我们还进行了四种不同的试验,即AmpC圆盘试验、改良的Hodge试验、改良的单琼脂板三维斑点接种试验(三合一)和硼酸抑制剂试验,以检测AmpC的产生。方法与材料:头孢西丁片筛选AmpC。采用AmpC圆盘试验、改良霍奇试验、三维斑点接种试验和硼酸圆盘试验检测耐头孢西丁菌AmpC的存在。统计分析:统计分析采用卡方检验和fisher精确检验,使用SPSS统计程序(20.0版)。结果:我院微生物科1年内共分离非重复革兰氏阴性杆菌1323株。在这1323株菌株中,发现150株菌株对头孢西丁和一种或多种第三代头孢菌素类抗生素耐药,或在头孢西丁和头孢噻肟片之间表现出拮抗,符合标准,纳入本研究。45例(3.4%)AmpC β内酰胺酶阳性。硼酸试验38例(25.3%),AmpC盘试验35例(23.3%),MHT 29例(19.3%),MTDST 27例(18%)。结论:硼酸抑制剂法检测出AmpC产生菌的最大数量。这是一个简单的和经济有效的测试,可以纳入常规药敏试验板,从而节省时间也。
Prevalence of AmpC Beta-lactamase in Gram Negative Bacilli by Different Phenotypic Methods in a Tertiary Care Institute in Kashmir
RS designed the study BAF managed the literature searches and analyses of the study performed. the experimental process. SL, A. Jan and A. Junaid in analyses of the results. SM in analysis of results and the first of the ABSTRACT Context: AmpC type cephalosporinases are Ambler class C β -lactamases. They hydrolyze penicillins, cephalosporins; except the fourth-generation compounds and monobactams. To the best of our knowledge prevalence of AmpC in our hospital is not known. Aims: With this background the study was undertaken to detect the prevalence of AmpC lactamases in gram-negative bacilli at our institute. We also performed four different tests, i.e. AmpC disc test, modified Hodge test, modified three dimensional spot inoculation test on a single agar plate (three in one) and inhibitor based test using boronic acid for detecting AmpC production. Methods and Materials: Screening for AmpC was done by using cefoxitin discs. Organisms resistant to cefoxitin were tested for presence of AmpC by AmpC disc test, Modified Hodge test, Three dimensional spot inoculation test and Boronic acid disc test. Statistical Analysis: Statistical analysis was done by Chi Square test and Fishers Exact Test with the SPSS statistical programme (version 20.0). Results: A total of 1323 non duplicate Gram negative bacilli were isolated during a period of one year in the Department of microbiology SKIMS. Out of these 1323 isolates, 150 isolates were found to be resistant to cefoxitin and one or more third generation cephalosporin antibiotics or showed antagonism between cefoxitin and cefotaxim discs thus fulfilling the criteria and were included in the present study. Forty five (3.4%) were positive for AmpC beta lactamases. Boronic acid test detected 38 (25.3%), AmpC disc test 35 (23.3%), MHT 29 (19.3%) and MTDST detected 27 (18%). Conclusions: Inhibitor based test using boronic acid detected the maximum number of AmpC producing organisms. It is a simple and cost effective test that can be incorporated in routine susceptibility testing plate thus saving time also.