双色鳗日粮赖氨酸需要量对生长激素相关基因表达的影响

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-08-29 DOI:10.36103/ijas.v54i4.1805

N. A. Prayogo, S. B. Prayitno, T. Winanto, T. Harisam, R. Fitriadi, B. Kusuma, P. Sukardi

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引用次数: 0

摘要

传统鳗鱼养殖户面临着商业玻璃鳗鱼饲料质量差、价格高的问题，这种饲料也产生了较差的鱼性能。缺乏必需氨基酸可能是原因。用分子方法测定玻璃鳗对配方饲料的快速反应。本研究的目的是确定在商品饲料中添加赖氨酸如何影响生长激素GH相关基因的表达。对玻璃鳗(1600头)饲喂面食饲料，培养60 d。圆柱形塑料池以钢为框架，覆盖HDPE塑料(300μm)，尺寸为{(3.14cm) x (85cmx85cm) x (60cm)}，填充水907,460 L≈0.91 m3。生长激素的实时RT-PCR结果表明，在8周的培养过程中，饲料中添加2%的赖氨酸水平对生长激素基因表达有很强的影响。

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LYSINE REQUIREMENT IN THE DIET OF GLASS EELS, ANGUILLA BICOLOR, AGAINST GENE EXPRESSION ASSOCIATED WITH GROWTH HORMONE

Traditional eel farmers face the problem of poor quality and high price of commercial glass eel diet, this feed has produced poor fish performance, as well. The lack of essential amino acids may be the cause. Molecular methods are used to determine the rapid response of glass eels to a formulated diet. The purpose of this study was to determine how the effect of adding lysine to commercial feed affects the expression of genes related to growth hormone GH. Pasta feed was given to glass eels (1600 heads) which were cultured for 60 days. The cylindrical plastic ponds were framed with steel and covered with HDPE plastic (300μm) in size {(3.14cm) x (85cmx85cm) x (60cm)} filled with water 907,460 L ≈ 0.91 m3. Real time RT-PCR results in GH suggest that the level of lysine (2%) added to the diet has strong effect on GH gene expression during eight weeks cultivation.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.