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引用次数: 1
摘要
摘要描述了一种定量分析生物样品中甘油脂分子种类的方法。1,2-二酰基- sn -甘油，无论是从生物样品中分离出来的，还是由磷酸甘油酯酶合成的，都是在sn -3位置进行苯甲酰化，然后进行反相(c18 -silica)高效液相色谱(HPLC)来分离不同疏水剂的分子种类。内标(1,2-二硬脂酰- sn -甘油)用于鉴定和定量从反相柱洗脱的各种物质。举例说明了SK-N-SH神经母细胞瘤细胞在受到细胞表面毒蕈碱乙酰胆碱受体刺激后所产生的甘油脂的分子种类和前体产物关系的定量分析。
Quantitative Analysis of Changes in the Molecular Species of Glycerolipids in Cultured Cells during Signal Transduction
Abstract A method for the quantitative analysis of the molecular species of glycerolipids present In biological samples has been described. 1,2-Diacyl- sn -glycerol, either isolated from biological samples or enzymatically generated from phosphoglycerides, is benzoylated at the sn -3 position and then subjected to reverse-phase (C 18 -silica) HPLC to separate the molecular species of different hydrophobicitles. An internal standard (1,2-distearoyl- sn -glycerol) is used to identify and quantify the various species eluted from the reverse-phase column. Examples are given for the quantitative analysis of molecular species and precursor-product relationships of glycerolipids generated In SK-N-SH neuroblastoma cells after stimulation of the cell-surface muscarinic acetylcholine receptors.
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