Koji Kato, Baoyao Wei, K. Matsunaga, R. Yamauchi, Y. Ueno
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引用次数: 0
摘要
从荞麦(Fagopyrum esculentum)胚乳中用冷水提取d -葡萄糖组成的多糖，通过DEAESephadex a -50 (C1-form)柱进行纯化，经ir分析、甲基化研究和酶解结果表明该多糖为植物糖原。凝胶色谱法测定多糖的平均分子量约为14,800。ƒÀ-Limit由多糖制备的糊精经污染酶和异淀粉酶消化。麦芽糖和麦芽糖与消化液中较高的低聚糖含量的比例支持了Meyer对这种多糖结构的模型。
Chemical Structure of α-D-Glucan in the Seed of Buckwheat(Fagopyrum esculentum)
The polysaccharide composed of only D-glucose was extracted with cold water from the endosperm of buckwheat (Fagopyrum esculentum) and purified through a column of DEAESephadex A-50 (C1-form), It was revealed to be a phytoglycogen from the results of IR-analysis , methylation study, and enzymic hydrolysis. The weight average molecular weight of the polysaccharide was determined to be about 14, 800 by gel chromatography. ƒÀ-Limit dextrin prepared from the polysaccharide was digested with pollulanase and then isoamylase. A ratio of maltose and maltotriose to higher oligosaccha rides in the digest supported Meyer's model for the structure of this polysaccharide .
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