临床样品中HIV-1前病毒DNA的自由标记电化学检测

A. Tamayo, L. S. L. Rizo, M. Armas, H. Yamanaka, A. M. E. Guas
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引用次数: 2

摘要

基于碳电极的DNA生物传感器正受到许多研究小组的大力研究。碳电极具有成本低、工作电位窗口宽、导电性好和背景电流相对较低等特点,在传感应用中具有特别的吸引力在过去的几年里,石墨在复合材料中的应用受到了极大的关注,利用其特殊的机械和电气性能来开发电化学平台。特别是,石墨复合材料在电化学过程的时间尺度内具有高的固有电阻和非常低的弛豫时间;因此,通过固体的渗透现象的参数和细节可以忽略石墨复合电极也可以很容易地修改,允许掺入不同的成分,如配体,酶,辅因子,介质,催化剂。因此,在开发可行的传感器来检测生物相关分子(如病毒DNA)时,使用这些电极具有特殊的兴趣。病毒性疾病的检测通常采用特定的技术,如免疫测定(ELISA)、聚合酶链反应(PCR)等然而,这些技术有几个缺点,例如需要昂贵的设备和试剂,涉及费力的程序,也需要有经验的人员。本研究的目的是用改性环氧石墨电极检测临床样品中的DNA,作为开发DNA病毒性疾病生物传感器的起始材料。复合电极的制备包括使用氧化石墨烯修饰环氧石墨,以引入生物相关物种潜在共价结合所需的羧基。最后的电极使用临床样本中的HIV-1前病毒DNA进行测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Free-label electrochemical detection of of HIV-1 proviral DNA in clinical sample
DNA biosensors based on carbon electrodes are under intense investigation by many research groups. Carbon electrodes are particularly attractive for sensing applications because of their low cost, wide working potential window, good electrical conductivity and relatively low background currents.1 A great deal of attention has given in the last years to the use of graphite in composite materials, taking advantage of their exceptional mechanical and electrical properties for the development of electrochemical platforms.2,3 In particular, graphite composites have high intrinsic resistance and very low relaxation time, within the time scale of the electrochemistry processes;4-6 thus, parameters and details about the percolation phenomena through the solid can be neglected.7 The graphite composite electrodes can also easily modify, allowing the incorporation of different components such as ligands, enzymes, cofactors, mediators, catalysts. Therefore, there is a special interest in using these electrodes in the development of feasible sensors for the detection of biologically-relevant molecules, such as DNA of viruses. The detection of viral diseases is usually performed by specific techniques such as immunoassay (ELISA), Polymerase Chain Reaction (PCR), among others.8 However, these techniques have several disadvantages, such as the need for expensive equipment and reagents involving laborious procedures that also requiring experienced personnel. The aim of this work was the detection of DNA in clinical sample with epoxy graphite electrodes modified as starting materials for the development of biosensors for DNA viral diseases. The composite electrode preparation included the use of epoxy graphite modified with graphene oxide, to introduce the carboxylic groups required for potential covalent binding of biologically relevant species. The final electrodes were test using a HIV-1 proviral DNA in clinical samples.
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