A. Tamayo, L. S. L. Rizo, M. Armas, H. Yamanaka, A. M. E. Guas
{"title":"临床样品中HIV-1前病毒DNA的自由标记电化学检测","authors":"A. Tamayo, L. S. L. Rizo, M. Armas, H. Yamanaka, A. M. E. Guas","doi":"10.15406/IJBSBE.2018.04.00129","DOIUrl":null,"url":null,"abstract":"DNA biosensors based on carbon electrodes are under intense investigation by many research groups. Carbon electrodes are particularly attractive for sensing applications because of their low cost, wide working potential window, good electrical conductivity and relatively low background currents.1 A great deal of attention has given in the last years to the use of graphite in composite materials, taking advantage of their exceptional mechanical and electrical properties for the development of electrochemical platforms.2,3 In particular, graphite composites have high intrinsic resistance and very low relaxation time, within the time scale of the electrochemistry processes;4-6 thus, parameters and details about the percolation phenomena through the solid can be neglected.7 The graphite composite electrodes can also easily modify, allowing the incorporation of different components such as ligands, enzymes, cofactors, mediators, catalysts. Therefore, there is a special interest in using these electrodes in the development of feasible sensors for the detection of biologically-relevant molecules, such as DNA of viruses. The detection of viral diseases is usually performed by specific techniques such as immunoassay (ELISA), Polymerase Chain Reaction (PCR), among others.8 However, these techniques have several disadvantages, such as the need for expensive equipment and reagents involving laborious procedures that also requiring experienced personnel. The aim of this work was the detection of DNA in clinical sample with epoxy graphite electrodes modified as starting materials for the development of biosensors for DNA viral diseases. The composite electrode preparation included the use of epoxy graphite modified with graphene oxide, to introduce the carboxylic groups required for potential covalent binding of biologically relevant species. The final electrodes were test using a HIV-1 proviral DNA in clinical samples.","PeriodicalId":15247,"journal":{"name":"Journal of Biosensors and Bioelectronics","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Free-label electrochemical detection of of HIV-1 proviral DNA in clinical sample\",\"authors\":\"A. Tamayo, L. S. L. Rizo, M. Armas, H. Yamanaka, A. M. E. Guas\",\"doi\":\"10.15406/IJBSBE.2018.04.00129\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"DNA biosensors based on carbon electrodes are under intense investigation by many research groups. Carbon electrodes are particularly attractive for sensing applications because of their low cost, wide working potential window, good electrical conductivity and relatively low background currents.1 A great deal of attention has given in the last years to the use of graphite in composite materials, taking advantage of their exceptional mechanical and electrical properties for the development of electrochemical platforms.2,3 In particular, graphite composites have high intrinsic resistance and very low relaxation time, within the time scale of the electrochemistry processes;4-6 thus, parameters and details about the percolation phenomena through the solid can be neglected.7 The graphite composite electrodes can also easily modify, allowing the incorporation of different components such as ligands, enzymes, cofactors, mediators, catalysts. Therefore, there is a special interest in using these electrodes in the development of feasible sensors for the detection of biologically-relevant molecules, such as DNA of viruses. The detection of viral diseases is usually performed by specific techniques such as immunoassay (ELISA), Polymerase Chain Reaction (PCR), among others.8 However, these techniques have several disadvantages, such as the need for expensive equipment and reagents involving laborious procedures that also requiring experienced personnel. The aim of this work was the detection of DNA in clinical sample with epoxy graphite electrodes modified as starting materials for the development of biosensors for DNA viral diseases. The composite electrode preparation included the use of epoxy graphite modified with graphene oxide, to introduce the carboxylic groups required for potential covalent binding of biologically relevant species. The final electrodes were test using a HIV-1 proviral DNA in clinical samples.\",\"PeriodicalId\":15247,\"journal\":{\"name\":\"Journal of Biosensors and Bioelectronics\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15406/IJBSBE.2018.04.00129\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biosensors and Bioelectronics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/IJBSBE.2018.04.00129","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Free-label electrochemical detection of of HIV-1 proviral DNA in clinical sample
DNA biosensors based on carbon electrodes are under intense investigation by many research groups. Carbon electrodes are particularly attractive for sensing applications because of their low cost, wide working potential window, good electrical conductivity and relatively low background currents.1 A great deal of attention has given in the last years to the use of graphite in composite materials, taking advantage of their exceptional mechanical and electrical properties for the development of electrochemical platforms.2,3 In particular, graphite composites have high intrinsic resistance and very low relaxation time, within the time scale of the electrochemistry processes;4-6 thus, parameters and details about the percolation phenomena through the solid can be neglected.7 The graphite composite electrodes can also easily modify, allowing the incorporation of different components such as ligands, enzymes, cofactors, mediators, catalysts. Therefore, there is a special interest in using these electrodes in the development of feasible sensors for the detection of biologically-relevant molecules, such as DNA of viruses. The detection of viral diseases is usually performed by specific techniques such as immunoassay (ELISA), Polymerase Chain Reaction (PCR), among others.8 However, these techniques have several disadvantages, such as the need for expensive equipment and reagents involving laborious procedures that also requiring experienced personnel. The aim of this work was the detection of DNA in clinical sample with epoxy graphite electrodes modified as starting materials for the development of biosensors for DNA viral diseases. The composite electrode preparation included the use of epoxy graphite modified with graphene oxide, to introduce the carboxylic groups required for potential covalent binding of biologically relevant species. The final electrodes were test using a HIV-1 proviral DNA in clinical samples.