Initiation of Basidioma Formation of Rare and Medicinal Macromycetes in Pure Culture

More than 5000 species of Ascomycetes and Agaricales fungi are known nowadays. However, 30 species of them are intensively cultivated and have been used into the agriculture. One of the reasons of a small number of species that can be grown in industrial culture are unclear conditions for the basidiomes formation. Basidiomes and mycelium include various nutritional substances that are used in food, medicine, cosmetics, etc. Recently, the biosynthesis of fungal biologically active substances with immunomodulation, radioprotective, antitumor, antiviral and other properties is under investigation by many scientists. Only 15 % of all products of medical mushrooms are made from extracts of mycelium. Verification of conditions of mushroom cultivation will significantly expand the range of species that could serve as a potential object for biotechnology.29 species of macromycetes from the pure Cultures Collection of Fungi (FCKU) of Educational and Scientific Centre «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv were used in our experiment. The initiation basidiomes formation of 29 macromycetes on different substrates in pure culture was studied. The optimal substrate for the studied species was husk of sunflower seeds. Most fungi formed basidiomes on it. If mushrooms did not formed basidiomes on this substrate, they fastest it overgrown or developed primordia or sclerotium-shaped structures. The shaping of the basidiomes, primordia or sclerotia-shaped structures was observed in 28 species. Three of these species were listed in the Red Book of Ukraine (Grifola frondosa, Leucoagaricus barsii, Sparassis crispa) and others rare species for Ukraine (Ceriporia viridans, Hericium cirrhatum, Sarcodontia crocea, Sparassis laminosa).