薯蓣皂苷元对CRF大鼠心脏组织脂质、微量元素、分子变化及TNF-α、IL-6表达的影响

Jeganathan Manivannan, Janakiraman Shanthakumar, Krishnamoorthy Rajeshwaran, Pandiyan Arunagiri, Elumalai Balamurugan
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引用次数: 6

摘要

本研究的目的是评估薯蓣皂苷元(一种已证实的抗氧化剂)对慢性肾功能衰竭(CRF)引起的心脏氧化应激异常的影响。用含0.75%腺嘌呤的饲粮喂养雄性Wistar大鼠,并口服薯蓣皂苷元(每天40 mg/kg)诱导CRF。测定薯蓣皂苷元对心肌组织脂质、微量元素(铁、锌、镁、铜、锰)及心肌线粒体酶活性的影响。同时检测肿瘤坏死因子α (TNF-α)和白细胞介素6 (IL-6)的表达。采用傅里叶变换红外光谱(FTIR)分析心脏组织中氧化应激相关的分子变化。研究结果表明,薯蓣皂苷元可减轻CRF引起的组织脂质异常。各组心脏元素浓度均无变化,血浆锌有改变,薯蓣皂苷元对其无影响。心肌线粒体酶异常和促炎细胞因子表达也显著降低。最后,薯蓣皂苷元还降低了蛋白质的分子和结构变化。综上所述,具有抗氧化功能的薯蓣皂苷元具有改善心脏组织异常的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of diosgenin on cardiac tissue lipids, trace elements, molecular changes, TNF-α and IL-6 expression in CRF rats

The aim of the present study was to evaluate the effect of diosgenin a proven antioxidant on chronic renal failure (CRF) induced abnormalities linked with oxidative stress in heart. CRF was induced in male Wistar rats by feeding the animals with 0.75% adenine-containing diet and diosgenin was given orally (everyday at the dose of 40 mg/kg). Effect of diosgenin on cardiac tissue lipids, trace elements (iron, zinc, magnesium, copper and manganese) and activity of cardiac mitochondrial enzymes were assayed. Expression of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) was also assessed. The Fourier transform infrared spectroscopy (FTIR) analysis was employed to indicate the oxidative stress related molecular changes in heart tissue. The outcomes of the study indicated that, diosgenin reduces the tissue lipid abnormalities induced by CRF. Cardiac elemental concentration was not changed in all groups but the plasma zinc was altered and diosgenin have no effect on it. Cardiac mitochondrial enzymes abnormalities and proinflammatory cytokines expression was also significantly reduced by diosgenin. Finally, the molecular and structural changes of proteins were also reduced by diosgenin treatment. The overall study shows that diosgenin with antioxidant function have enough potential to improve cardiac tissue abnormalities.

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