芡实胚离体培养条件的优化。(Makhana)使用2,4 - d作为生长调节剂

Ramesh Kumar, M. Abdin, V. Jha, I. S. Singh, Amit Kumar
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引用次数: 2

摘要

以芡粉(Euryale ferox, Makhana)细胞为材料,通过合子胚胎发生,优化了高频植物调控的培养条件。合子胚发育成淡黄色的球状结构和小愈伤组织,然后形成芽,频率为40%。在半强度的Murashige和Skoog (MS)培养基上,添加0、0.1、0.3、0.5、1.0、1.5 mg/L的2,4- d作为生长调节剂培养时,呈淡黄色球状结构的频率呈弹性增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of in vitro conditions for embryo culture of Euryale ferox Salisb. (Makhana) using 2, 4-D as growth regulator
Culture conditions were optimized for high-frequency plant regulation via zygotic embryogenesis from cell culture of  Euryale ferox  (Makhana). Zygotic embryo developed into the yellowish globular structure and small callus followed by shoot formation at a frequency of 40%. When cultured on half-strength Murashige and Skoog (MS) medium, supplemented with 0, 0.1, 0.3, 0.5, 1.0, 1.5 mg/L of 2,4-D as growth regulator, the frequency of yellowish globular structure increased flabbily.
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