海藻酸盐-壳聚糖膜固定化脲酶的聚电解质复合物

IF 0.5 Q4 MULTIDISCIPLINARY SCIENCES

Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-20 DOI:10.5614/j.math.fund.sci.2019.51.3.8

D. Hermanto, M. Mudasir, D. Siswanta, B. Kuswandi, N. Ismillayli

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引用次数: 17

摘要

制备了海藻酸盐-壳聚糖膜作为固定化脲酶的载体材料。本研究旨在开发一种稳定性高、反应时间快、制备工艺简单且相对便宜的固定化酶载体材料。将海藻酸纯溶胶与壳聚糖(质量比为1:1)在pH 5.28条件下反应，混合后室温干燥制得海藻酸-壳聚糖PEC膜。FTIR、XRD和SEM分析证实得到了海藻酸-壳聚糖PEC。BTB指示剂的颜色变化证明脲酶被困在海藻酸-壳聚糖PEC膜的空腔中，而固定化脲酶仍具有催化活性。因此，海藻酸-壳聚糖PEC膜作为脲酶固定化基质具有良好的性能。

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Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.

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来源期刊

Journal of Mathematical and Fundamental Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： Journal of Mathematical and Fundamental Sciences welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Astronomy, Chemistry, Earth Sciences (Geodesy, Geology, Geophysics, Oceanography, Meteorology), Life Sciences (Agriculture, Biochemistry, Biology, Health Sciences, Medical Sciences, Pharmacy), Mathematics, Physics, and Statistics. New submissions of mathematics articles starting in January 2020 are required to focus on applied mathematics with real relevance to the field of natural sciences. Authors are invited to submit articles that have not been published previously and are not under consideration elsewhere.