金属对硝酸杆菌氧吸收、二氧化碳固定和细胞色素还原的影响

Y. Tsai, O. Tuovinen
{"title":"金属对硝酸杆菌氧吸收、二氧化碳固定和细胞色素还原的影响","authors":"Y. Tsai, O. Tuovinen","doi":"10.1002/TOX.2540040207","DOIUrl":null,"url":null,"abstract":"Oxygen uptake coupled with nitrite oxidation by washed cell suspensions of Nitrobacter agilis was tested in the presence of copper, nickel, aluminum, uranyl, and molybdate ions. Copper ion was slightly stimulatory at low concentrations and strongly inhibitory at 17 mM. Molybdate ion showed either slight enhancement or no inhibition at all test concentrations. With the other test ions, inhibition of oxygen uptake was observed. Carbon dioxide fixation was generally more sensitive to metal ions than was oxygen uptake. Cytochrome reduction in washed cells of N. agilis was demonstrated in the presence and absence of metal ions. Cytochrome aa3 and cytochrome a1 (α-peak at 590 nm) were sensitive to aluminum, uranyl, and molybdate ions, whereas cytochrome c (β-peak at 520 nm) was insensitive to test ions. All metal ions inhibited the reduction of cytochrome a1 (γ-peak at 438 nm). This inhibition decreased when the concentration of copper and molybdate was increased to 1.7 mM. Only aluminum and nickel ions demonstrated a concentration-dependent inhibition of cytochrome a1 (438 nm). The test ions blocked the activity of cytochrome c (α-peak at 550 nm). The inhibition of cytochrome c at 415 nm (γ-peak) was also apparent.","PeriodicalId":11824,"journal":{"name":"Environmental Toxicology & Water Quality","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Influence of metals on oxygen uptake, carbon dioxide fixation, and cytochrome reduction in Nitrobacter Agilis\",\"authors\":\"Y. Tsai, O. Tuovinen\",\"doi\":\"10.1002/TOX.2540040207\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Oxygen uptake coupled with nitrite oxidation by washed cell suspensions of Nitrobacter agilis was tested in the presence of copper, nickel, aluminum, uranyl, and molybdate ions. Copper ion was slightly stimulatory at low concentrations and strongly inhibitory at 17 mM. Molybdate ion showed either slight enhancement or no inhibition at all test concentrations. With the other test ions, inhibition of oxygen uptake was observed. Carbon dioxide fixation was generally more sensitive to metal ions than was oxygen uptake. Cytochrome reduction in washed cells of N. agilis was demonstrated in the presence and absence of metal ions. Cytochrome aa3 and cytochrome a1 (α-peak at 590 nm) were sensitive to aluminum, uranyl, and molybdate ions, whereas cytochrome c (β-peak at 520 nm) was insensitive to test ions. All metal ions inhibited the reduction of cytochrome a1 (γ-peak at 438 nm). This inhibition decreased when the concentration of copper and molybdate was increased to 1.7 mM. Only aluminum and nickel ions demonstrated a concentration-dependent inhibition of cytochrome a1 (438 nm). The test ions blocked the activity of cytochrome c (α-peak at 550 nm). The inhibition of cytochrome c at 415 nm (γ-peak) was also apparent.\",\"PeriodicalId\":11824,\"journal\":{\"name\":\"Environmental Toxicology & Water Quality\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Toxicology & Water Quality\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/TOX.2540040207\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Toxicology & Water Quality","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/TOX.2540040207","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

摘要

在铜、镍、铝、铀酰和钼酸盐离子存在的情况下,对敏捷硝化杆菌洗涤细胞悬浮液的氧摄取耦合亚硝酸盐氧化进行了测试。铜离子在低浓度下有轻微的刺激作用,在17 mM时有较强的抑制作用。钼酸盐在所有浓度下均有轻微的增强作用或无抑制作用。与其他测试离子,观察到氧摄取的抑制。二氧化碳固定通常对金属离子比氧摄取更敏感。在存在和不存在金属离子的情况下,细胞色素在洗涤后的细胞中减少。细胞色素aa3和细胞色素a1 (α-峰在590 nm处)对铝、铀酰和钼酸盐离子敏感,而细胞色素c (β-峰在520 nm处)对测试离子不敏感。所有金属离子均抑制细胞色素a1的还原(γ-峰在438 nm处)。当铜和钼酸盐浓度增加到1.7 mM时,这种抑制作用减弱。只有铝和镍离子对细胞色素a1表现出浓度依赖性的抑制作用(438 nm)。实验离子阻断细胞色素c的活性(α-峰在550 nm处)。在415 nm (γ峰)处对细胞色素c的抑制作用也很明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of metals on oxygen uptake, carbon dioxide fixation, and cytochrome reduction in Nitrobacter Agilis
Oxygen uptake coupled with nitrite oxidation by washed cell suspensions of Nitrobacter agilis was tested in the presence of copper, nickel, aluminum, uranyl, and molybdate ions. Copper ion was slightly stimulatory at low concentrations and strongly inhibitory at 17 mM. Molybdate ion showed either slight enhancement or no inhibition at all test concentrations. With the other test ions, inhibition of oxygen uptake was observed. Carbon dioxide fixation was generally more sensitive to metal ions than was oxygen uptake. Cytochrome reduction in washed cells of N. agilis was demonstrated in the presence and absence of metal ions. Cytochrome aa3 and cytochrome a1 (α-peak at 590 nm) were sensitive to aluminum, uranyl, and molybdate ions, whereas cytochrome c (β-peak at 520 nm) was insensitive to test ions. All metal ions inhibited the reduction of cytochrome a1 (γ-peak at 438 nm). This inhibition decreased when the concentration of copper and molybdate was increased to 1.7 mM. Only aluminum and nickel ions demonstrated a concentration-dependent inhibition of cytochrome a1 (438 nm). The test ions blocked the activity of cytochrome c (α-peak at 550 nm). The inhibition of cytochrome c at 415 nm (γ-peak) was also apparent.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信