衣壳修饰蓝舌病毒16 (BTV16)作为抗病毒肿瘤治疗剂的研究

Biotechnology and Health Sciences Pub Date : 2017-03-09 DOI:10.5812/BHS.45667

T. Farivar, R. Najafipour, S. Alizadeh, S. M. Azimi, P. Johari

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引用次数: 0

摘要

目的:利用潜在病毒摧毁癌细胞已有很长的历史，但最近分子生物学的进展为成功利用这些病毒再次带来了希望。方法:将Octreotate序列插入BTV 10段基因组衣壳片段vp2蛋白中和区(R1& R2) 304 ~ 368位。提取T7 BTV RNA转录本。用野生和修饰的BTV转染癌细胞，用dna衍生的基因组片段恢复BTV。结果:所有实验结果表明，用VP2修饰的靶向癌细胞表面的BTV16处理AGS细胞系，可显著增加癌感染细胞的凋亡。结论:修饰后的VP2 BTV16可作为AGS细胞的抗病毒肿瘤治疗药物。关键词:蓝舌病毒;肿瘤治疗;毒解

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Capsid Modified Bluetongue Virus 16 (BTV16) as a Virulytic Oncotherapy Agent

Objective: Using potential viruses to destroy cancer cells has a long history, but recent advances in molecular biology raised hopes for successful use of these viruses again. Methods: Octreotate sequence was inserted into the neutralization region (R1& R2) in vp2 protein of capsid segment in 10 segmented genome of BTV in 304 - 368 position. T7 BTV RNA transcripts were extracted. Cancerous cultured cells were transfected with wild and modified BTV to recover BTV with cDNA-derived genome segments. Results: The results of all the performed experiments revealed that treatment of AGS cell lines with VP2 modified BTV16, which targeted cell surface of cancerous cells, significantly increased apoptosis in cancer infected cells. Conclusions: Modified VP2 BTV16 may be used as a potential virulytic oncotherapy agent in AGS cells. Keywords: Bluetongue Virus, Oncotherapy, Virulytic, AGS

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Biotechnology and Health Sciences

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