{"title":"癌症患者游离DNA中癌症相关基因的DNA甲基化分析","authors":"Yukie Saeki , Minako Abe , Fumika Kono , Akane Nakazato , Yoshimori Ishihara , Hiroyuki Abe","doi":"10.1016/j.pmu.2017.04.002","DOIUrl":null,"url":null,"abstract":"<div><p>DNA methylation<span><span> is a very important mechanism for regulating gene expression in normal development and cell differentiation. However, DNA methylation, when acting on the promoter region of a </span>tumor suppressor gene<span>, suppresses transcription and is one of the major epigenetic modifications involved in the development and progression of many cancers (Wajed et al., 2001; Morris and Chan 2015). In our laboratory, we have carried out methylation analysis of 14 different tumor suppressor genes in cell-free DNA from the peripheral blood of over 1400 cancer patients, and have detected more than a total of 1900 methylations. We performed an analysis of DNA methylations of 14 different tumor suppressor genes, by type of cancer, stage of cancer, function of tumor suppressor genes, and their correlation to genetic mutations.</span></span></p></div>","PeriodicalId":101009,"journal":{"name":"Personalized Medicine Universe","volume":"6 ","pages":"Pages 4-11"},"PeriodicalIF":0.0000,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.pmu.2017.04.002","citationCount":"1","resultStr":"{\"title\":\"DNA methylation analysis of cancer-related genes from cell-free DNA of patients with cancer\",\"authors\":\"Yukie Saeki , Minako Abe , Fumika Kono , Akane Nakazato , Yoshimori Ishihara , Hiroyuki Abe\",\"doi\":\"10.1016/j.pmu.2017.04.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>DNA methylation<span><span> is a very important mechanism for regulating gene expression in normal development and cell differentiation. However, DNA methylation, when acting on the promoter region of a </span>tumor suppressor gene<span>, suppresses transcription and is one of the major epigenetic modifications involved in the development and progression of many cancers (Wajed et al., 2001; Morris and Chan 2015). In our laboratory, we have carried out methylation analysis of 14 different tumor suppressor genes in cell-free DNA from the peripheral blood of over 1400 cancer patients, and have detected more than a total of 1900 methylations. We performed an analysis of DNA methylations of 14 different tumor suppressor genes, by type of cancer, stage of cancer, function of tumor suppressor genes, and their correlation to genetic mutations.</span></span></p></div>\",\"PeriodicalId\":101009,\"journal\":{\"name\":\"Personalized Medicine Universe\",\"volume\":\"6 \",\"pages\":\"Pages 4-11\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.pmu.2017.04.002\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Personalized Medicine Universe\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2186495017300032\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Personalized Medicine Universe","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2186495017300032","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
DNA甲基化是调控正常发育和细胞分化过程中基因表达的重要机制。然而，当DNA甲基化作用于肿瘤抑制基因的启动子区域时，会抑制转录，并且是许多癌症发生和进展中涉及的主要表观遗传修饰之一(Wajed等人，2001;Morris and Chan 2015)。在我们的实验室中，我们对1400多名癌症患者外周血的游离DNA中14种不同的肿瘤抑制基因进行了甲基化分析，共检测到1900多个甲基化。我们根据癌症类型、癌症分期、肿瘤抑制基因的功能及其与基因突变的相关性，对14种不同肿瘤抑制基因的DNA甲基化进行了分析。
DNA methylation analysis of cancer-related genes from cell-free DNA of patients with cancer
DNA methylation is a very important mechanism for regulating gene expression in normal development and cell differentiation. However, DNA methylation, when acting on the promoter region of a tumor suppressor gene, suppresses transcription and is one of the major epigenetic modifications involved in the development and progression of many cancers (Wajed et al., 2001; Morris and Chan 2015). In our laboratory, we have carried out methylation analysis of 14 different tumor suppressor genes in cell-free DNA from the peripheral blood of over 1400 cancer patients, and have detected more than a total of 1900 methylations. We performed an analysis of DNA methylations of 14 different tumor suppressor genes, by type of cancer, stage of cancer, function of tumor suppressor genes, and their correlation to genetic mutations.
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