S. Barreca, M. Busetto, Carola Forni, Luisa Colzani, Laura Clerici, Daniela Daverio, S. Balzamo, E. Calabretta, M. Peleggi, P. Dellavedova
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引用次数: 3
摘要
近年来,水生生态系统的质量越来越受到欧洲机构的关注。欧盟委员会实施决定(EU) 2018/840起草了一份在欧洲监测的化合物观察清单(WL)。在本研究中,我们报告了一种基于超高效液相色谱固相萃取,结合三重四极杆质谱仪(UHPLC-MS/MS)对整个水样进行分析的方法。建立并验证了该方法对12个所列化合物的测定。采用水平系统的固相萃取确保了对整个样品的分析,并最大限度地减少了样品操作。不同的ng L−1检测限(从2到50 ng L−1)、线性(从2到500 ng L−1)、准确度(从70到130%)和精密度水平(在loq水平上RSD小于20%)被评估为在感兴趣的水平上令人满意的定量和确认。在2019年和2020年区域环境保护机构的监测活动中,开发的方法用于定量分析意大利不同地点地表水样品中的观察名单化合物(激素除外)。
Determination of Antibiotics, Pesticides, Herbicides, Fungicides and Hormones in Water Bodies in Italy in Occurrence with European Watch List Mechanism by Using an UHPLC-MS/MS System: Method Validation, Quantification and Evaluations
In recent years, the quality of aquatic ecosystems has received increasing attention from European institutions. The Commission Implementing Decision (EU) 2018/840 drafted a Watch List (WL) of compounds to be monitored in Europe. In this study, we report a method based on solid phase extraction with ultra-high-performance liquid chromatography, coupled with a triple-quadrupole mass spectrometer (UHPLC-MS/MS) to analyze the whole water sample. The method was developed and validated for the determination of 12 listed compounds. The employment of solid-phase extraction by a horizon system ensures the analysis of the entire body of samples and minimizes sample manipulation. Different ng L−1 detection limits (from 2 to 50 ng L−1), linearities (from 2 to 500 ng L−1), accuracy (from 70 to 130%) and levels of precision (RSD less 20% at LOQs levels) were assessed to be satisfactory for quantification and confirmation at the levels of interest. The developed method was applied for quantitative analysis for Watch List compounds (with the exception of hormones) in surface water samples from different Italian sites during monitoring activities by the Regional Environmental Protection Agencies in the years 2019 and 2020.