水提物急性毒性的评价

R. Chalannavar, S DivakarM, Souvik Debnath, T. Chatterjee, Sooryaprakash H Shenoy
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引用次数: 1

摘要

一种稀有植物——桃金娘科(桃金娘科)。本研究对kanarense叶片水提物(AESKL)的初步植物化学筛选、体外毒性和急性毒性进行了评价。以NIH/3T3细胞株为实验材料,筛选植物化学物质,并采用MTT法检测细胞毒性。急性毒性评价,雌性大鼠分为两组;一组作为对照组,另一组以2000mg/kg体重(b.w)单剂量给药,治疗观察14 d。AESKL含有酚类物质、单宁、萜类、皂苷和类固醇。结果表明,AESKL的抑制浓度(IC50) = 491.56µg/ml。AESKL的急性毒性确定致死剂量(LD50)大于2000 mg/kg b.w,未观察到死亡率。行为学、体重、相对脏器重量、血液学、生化和组织学均无变化。综上所述,大鼠口服AESKL无风险。据我们所知,这是第一篇关于AESKL体外和体内毒性评价的报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The EVALUATION OF ACUTE TOXICITY EXPOSURE TO AQUEOUS LEAVES EXTRACT OF SYZYGIUM KANARENSE (TALBOT) RAIZADA
Syzygium kanarense (Talbot) Raizada (Family: Myrtaceae), a rare plant tested for toxicity in order to explore therapeutic value. In the current study, the preliminary phytochemical screening, in vitro toxicity and acute toxicity of aqueous extract of Syzygium kanarense leaves (AESKL) were evaluated. Phytochemicals were screened and cytotoxicity was confirmed by MTT assay using NIH/3T3 cell line. For acute toxicity evaluation, female rats were divided into two equal groups; one group served as control while the other group received a single dose of AESKL at 2000mg/kg body weight (b.w) were treated and observed for 14 days. AESKL contains phenolics, tannins, terpenoids, saponins and steroids. The result showed the cytotoxicity value of inhibitory concentration (IC50) = 491.56 µg/ml of AESKL. Acute toxicity of AESKL established the Lethal Dose (LD50) more than 2000 mg/kg b.w and no mortality rate was observed. No changes in behavioural, b.w, relative organ weight, haematological, biochemical and histological were observed. Based on the results, it can be conclude that AESKL do not pose any risk when given orally to rats. To the best of our knowledge, this is the first report on evaluation of in vitro and in vivo toxicity of AESKL.
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