利用标记辅助育种方法进行耐旱性分子评价

F. Fatimah, J. Prasetiyono, K. Trijatmiko, Sustiprijatno Sustiprijatno
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引用次数: 1

摘要

国家稻米生产的可持续性和增加需要粮食和农业部门准备好应对气候变化、土地退化、干旱地区、倾斜生产和人口增长加快的影响。适应在确保粮食安全的可持续性方面发挥着重要作用。本研究旨在利用标记辅助回交技术,通过对已鉴定的前景选择qtl进行基因金字塔化,培育耐旱品种Inpari 30和Situ Bagendit,并探索分布在耐旱供体(Cabacu)和受体水稻(Inpari 30和Situ Bagendit) 12条水稻染色体上的SSRs和6K snp用于背景选择。前景选择结果显示,qRPF2.1、qGPP2.1、qSPP4.1和Sub1四个qtl的侧翼ssr均小于2 cM。通过水稻6K SNP引物多态性调查进行背景选择,Inpari 30与Cabacu的多态性位点为2457个(53.3%),原位Bagendit与Cabacu的多态性位点为2563个(55.6%),平均距离约为0.74 cM/染色体。F1 Inpari 30/Cabacu和F1 Situ Bagendit/Cabacu这4个qtl的基因型选择均已达到杂合子条件。将这些系进行回交育种,获得BC1F1 Inpari 30/Cabacu代和BC1F1 Situ Bagendit/Cabacu代。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Evaluation for Drought Tolerant Using Marker Assisted Breeding Method
   The sustainability and increasing the national rice production require the readiness of food and agriculture sector cope with the impacts of climate change, land degradation, drought area, sloping production and the raising of population growth. Adaptation plays an important role in ensuring the sustainability of food security. This research aimed to develop drought-tolerant variety of Inpari 30 (submergence tolerance variety) and Situ Bagendit through marker-assisted backcrossing-through pyramiding gene of identified QTLs for foreground selection and to explore SSRs and 6K SNPs for background selection distributed in 12 rice chromosome of drought tolerant donor (Cabacu) and recipient rice (Inpari 30 and Situ Bagendit). The foreground selection revealed that flanking SSRs of each QTLs (qRPF2.1, qGPP2.1, qSPP4.1 and Sub1) was less than 2 cM. The background selection through polymorphic survey of Rice 6K SNP primers revealed 2457 (53,3%) polymorphic SNPs on Inpari 30 vs Cabacu and 2563 (55,6%) polymorphic SNPs on Situ Bagendit vs Cabacu with the average distance about 0.74 cM/chromosome. The genotypic selection of F1 Inpari 30/Cabacu and F1 Situ Bagendit/Cabacu have already in heterozygote condition for these 4 QTLs target. These lines was continued for backcross breeding to develop BC1F1 Inpari 30/Cabacu and BC1F1 Situ Bagendit/Cabacu generation.
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