人类真菌病原体马尔尼菲青霉的分子遗传操作和可视化策略

Fungal Genetics Reports Pub Date : 2012-05-01 DOI:10.4148/1941-4765.1009

Kylie J. Boyce, Hayley E. Bugeja, Harshini Weerasinghe, Michael Payne, Lena Schreider

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引用次数: 13

摘要

马尼菲拟虫已成为研究其形态发生和致病性的实验对象。本文描述了一些工具的发展，包括许多可选择的标记，以扩大它可以被基因操纵的便利性。结合外源DNA同源重组工程菌株，这些工具促进了高效的分子遗传学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Strategies for the molecular genetic manipulation and visualization of the human fungal pathogen Penicillium marneffei

P. marneffei has been established as an experimentally amenable system to study morphogenesis and pathogenicity. This paper describes the development of a number of tools, including numerous selectable markers, to expand the ease with which it can be genetically manipulated. Combined with strains engineered for homologous recombination of exogenous DNA, these tools facilitate efficient molecular genetic studies.

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Fungal Genetics Reports

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