微室阵列微细胞包封用海藻酸盐均匀胶囊的批量生产

H. Kitagawa, Wei-heong Tan, S. Takeuchi
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引用次数: 2

摘要

这项工作的动机是需要将贴壁细胞和非贴壁细胞精确定位到阵列中,这些阵列可以在高通量筛选(HTS)中与微流控通道集成,用于药物发现。我们描述了一种微流体装置,可以快速生产均匀的微水凝胶胶囊,可以包含细胞。在10毫米长的微通道中使用微室可以同时简单地形成和排列多达1times104个水凝胶胶囊。本研究以海藻酸盐为水凝胶胶囊,采用EDTA溶解去除;因此,在阵列中观察后,可以收集密闭在胶囊中的样品。我们演示了将大肠杆菌微胶囊化成海藻酸盐胶囊,并成功地将胶囊与EDTA溶解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mass production of uniform alginate capsules for micro cell encapslation using micro chamber array
This work is motivated by the need for accurate positioning of both adherent and non-adherent cells into arrays that can be integrated with microfluidic channels in high-throughput screening (HTS) for drug discovery. We describe a microfluidic device that allows rapid production of uniform micro hydrogel capsules that can contain cells. Up to 1times104 hydrogel capsules can be simply formed and arrayed simultaneously using microchambers in a 10 mm long microchannel. In this work, alginate was used as the hydrogel capsules that can be dissolved and removed with EDTA; thus samples confined in the capsules are collectable after observation in the array. We demonstrated the microencapsulation of E. coli into alginate capsules, and dissolved the capsules with EDTA successfully.
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