Pharmacological investigation of leaves of Polypodium decumanum for anti-bacterial activity against gram-positive and gram-negative bacteria

http://dx.doi.org/10.21276/IJRDPL.227 8-0238.2017.6(4).2685-2688 ABSTRACT:The widespread use of antibiotics has led to an increase in multidrugresistant bacteria, which represent a serious risk of infection, in view of this problem the aim of this research was performed to characterize the antibacterial effect of ethanolic and petroleum ether extract of Polypodium decumanum leaves against three gram-positive bacteria(Staphylococcus aureus, Bacillus subtilius, and Streptococcus pyogenes) and three gram-negative bacteria(Pseudomonas aeruginosa, Escherichia coli, and Pseudomonas alcaligenes) in vitro by disc-diffusion method. In this study, we have taken 3 concentrations of Polypodium decumanumplant extract (ethanolic and petroleum ether) viz. 50 mg/ml, 100 mg/ml, and 200 mg/ml, and observed that the ethanolic extract of Polypodium decumanumof the concentration of 200 mg/ml showed higher antibacterial effect compare to other concentration against all 3 gram-positive bacteria and all 3 gram-negative bacteria, but not higher than standard drug Ampicillin.