几种植物提取物对寻常痤疮患者分离细菌的抑菌活性研究

K. Rasool
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There are different ways to treat acne such as  using of antibiotics  , herpes , and mixing treatments . \nMethods : Antibacterial activity  of  four concentrations (100,50,25,12.5)mg /ml of  alcoholic  and cold  aqueous  crude extracts of Cinnamon(Cinnamomum verum ), Henna (Lawsonia inermis ) , Lupine (Lupinus luteus) were studied against aerobic and  an aerobic bacteria isolated from inflamed and discharging pus  from thirty Iraqi acne vulgaris patients refer to dermatology unit  at AL-Kindy Hospital from December 2016  to March 2017.All information (age, sex ,diseases and using topical treatments) were recorded .The bacterial isolates  were identify using morphological characteristics, biochemical tests and  the  Vitek-2 compact system. \nResults: Among (30 )Acne samples taken, 8 (26.7%) samples were from males age range (19-33) years and 22(73.3%) were from females within age (17-29)years . twenty five (83%) samples  were culture positive, and only  (17%) of samples revealed no growth .Most frequent  bacteria which isolated  (aerobically) from acne patients were  Staphylococcus aureus ( 60%), Staphylococcus epidermidis (20%),  Escherichia coli  (8% ),  Pseudomonas aeruginosa  (4%), and an aerobic bacterial isolates  were Propionibacterium acnes  (8%) isolates. \n  \nAntibiotic sensitivity tests were performed against, Ampicillin, Clindamycin, Gentamicin, Cotrimoxazole, Erythromycin, Vancomicine, Tetracycline, Doxycycline, and Azithromycin.All bacterial isolates were resistance to Ampicillin. 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引用次数: 2

摘要

背景:痤疮是一种皮肤多形性紊乱皮肤病,最常见于12-24岁的青少年,估计百分比为85%。治疗痤疮有不同的方法,如使用抗生素、疱疹和混合治疗。方法:研究肉桂(Cinnamomum verum)、指甲花(Lawsonia inermis)、Lupine (Lupinus luteus)四种浓度(100、50、25、12.5)mg /ml的乙醇和冷水粗提物对30例伊拉克寻常痤疮患者炎症和排出脓液中分离的好氧菌和一种好氧菌的抑菌活性。记录所有信息(年龄,性别,疾病和使用局部治疗)。采用形态学特征,生化试验和Vitek-2紧凑系统鉴定分离的细菌。结果:30例痤疮样本中,男性(19 ~ 33岁)8例(26.7%),女性(17 ~ 29岁)22例(73.3%)。25份(83%)样本培养阳性,只有17%的样本未见生长。从痤疮患者中分离出的需氧细菌最常见的是金黄色葡萄球菌(60%)、表皮葡萄球菌(20%)、大肠杆菌(8%)、铜绿假单胞菌(4%),需氧细菌分离物为痤疮丙酸杆菌(8%)分离物。对氨苄西林、克林霉素、庆大霉素、复方新诺明、红霉素、万古霉素、四环素、多西环素和阿奇霉素进行抗生素敏感性试验。所有细菌分离株均对氨苄西林耐药。金黄色葡萄球菌和表皮葡萄球菌对多西环素和阿奇霉素敏感(100%),痤疮假单胞菌对这两种抗生素也高度敏感(分别为95.5%和97.1%),大肠杆菌和铜绿假单胞菌对这两种抗生素均耐药(100%)。除铜绿假单胞菌(P. aeruginosa)对CN和TE极耐外,其余菌株均对庆大霉素和四环素敏感(分别为100%和94.8%)。采用孔扩散法测定了浓度为(100、50、25、12.5)mg/ml的肉桂(Cinnamomum verum)、月桂(Lawsonia inermis)和羽扇豆(Lupinus luteus)的冷水和酒精粗提物对革兰氏阳性和革兰氏阴性分离株的抑菌作用。指甲花叶醇提物浓度(100mg/ml)对所有菌株的抑菌活性均高于水提物和肉桂醇提物浓度(100mg/ml),而羽扇豆醇提物对所有菌株均无抑制作用。结论:革兰氏阳性菌是痤疮感染最常见的微生物。痤疮感染的发病率与性别、年龄有关;指甲花叶的酒精提取物可用于治疗痤疮。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial activity of some plants extracts on bacteria isolated from acne vulgaris patients
Background: Acne is a cutaneous pleomorphic disorder skin disease most frequently occurring during the adolescent in ages of 12-24, with estimated  percentage ( 85%) . There are different ways to treat acne such as  using of antibiotics  , herpes , and mixing treatments . Methods : Antibacterial activity  of  four concentrations (100,50,25,12.5)mg /ml of  alcoholic  and cold  aqueous  crude extracts of Cinnamon(Cinnamomum verum ), Henna (Lawsonia inermis ) , Lupine (Lupinus luteus) were studied against aerobic and  an aerobic bacteria isolated from inflamed and discharging pus  from thirty Iraqi acne vulgaris patients refer to dermatology unit  at AL-Kindy Hospital from December 2016  to March 2017.All information (age, sex ,diseases and using topical treatments) were recorded .The bacterial isolates  were identify using morphological characteristics, biochemical tests and  the  Vitek-2 compact system. Results: Among (30 )Acne samples taken, 8 (26.7%) samples were from males age range (19-33) years and 22(73.3%) were from females within age (17-29)years . twenty five (83%) samples  were culture positive, and only  (17%) of samples revealed no growth .Most frequent  bacteria which isolated  (aerobically) from acne patients were  Staphylococcus aureus ( 60%), Staphylococcus epidermidis (20%),  Escherichia coli  (8% ),  Pseudomonas aeruginosa  (4%), and an aerobic bacterial isolates  were Propionibacterium acnes  (8%) isolates.   Antibiotic sensitivity tests were performed against, Ampicillin, Clindamycin, Gentamicin, Cotrimoxazole, Erythromycin, Vancomicine, Tetracycline, Doxycycline, and Azithromycin.All bacterial isolates were resistance to Ampicillin. Staphylococcus aureus and S. epidermidis were sensitive (100%) to Doxycycline and Azithromycin, P. acne were also highly sensitive to these two antibiotics (95.5%,97.1%) respectively, while E. coli and P. aeruginosa were (100%) resistance to these antibiotics. Gentamicin and tetracycline were susceptible by most of the study isolates except for P. aeruginosa which was very resistance to CN and TE (100%&94.8%) respectively. The antimicrobial potential of cold water and alcoholic crude extracts of Cinnamon (Cinnamomum verum ), Hinna (Lawsonia inermis), and Lupine (Lupinus luteus), in concentrations (100,50,25,12.5) mg/ml against the gram positive and gram negative isolates were tested through a well-diffusion method. Alcoholic extract of  Henna leaves in concentration  (100mg/ml) showed high inhibitory activity  to all isolates compared with the aqueous extract and the all concentration of cinnamon aqueous and alcoholic extracts ,while  lupine extracts  had no effect on all  bacterial isolates   . Conclusion: Gram-positive bacteria were the most common microorganisms involved  in  acne infection. There are variations in the incidence of acne infection in relation to sex, age; Alcoholic extract of the Henna leaves could be used to treat acne.
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