几种地方植物脲酶提取工艺条件的确定

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-06-25 DOI:10.36103/ijas.v54i3.1776

Amaal A. H, Sahar I. H, Mohanad J. M-Ridha

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引用次数: 1

摘要

本研究选取了鹰嘴豆、番茄、大豆、芥菜、贝克菜、Lebbeck、豆类、芝麻、伊拉克公莓、伊拉克母莓、印度莓、马铃薯、萝卜、豆类、豌豆、西瓜和菜豆等17种常用植物进行脲酶活性筛选，其中芝麻酶活性最高，脲酶产量最高(1.623 U/mg蛋白)。最佳提取比为1:10 (W: V)，提取时间为90 min，蛋白质提取率为0.8414U/mg。选择磷酸钠缓冲液(0.1 M, pH 7.0)为最佳提取缓冲液，比活性为0.9004U/mg蛋白。

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DETERMINATION OF THE OPTIMUM CONDITIONS FOR UREASE EXTRACTED FROM SOME LOCAL PLANTS

In the current study, Seventeen types of plants commonly used namely (Chickpeas, Tomato, Soybean, Mustard, Baker, Lebbeck, Bean, Sesame, Male Iraqi berries, Female Iraqi berries, Indian berries, Potato, Radish, Legumes, peas, Watermelon, and Phaseolus were obtained and screened for urease activity, among these plants, Sesame was chosen with maximum enzymatic activity, and it had the highest productivity of urease enzyme (1.623 U/mg protein). The optimum extraction ratio represented by 1:10 (W: V) after 90 minutes and 0.8414U/mg protein. Sodium phosphate buffer (0.1 M, pH 7.0) was chosen as the best extraction buffer with specific activity 0.9004U/mg protein.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.