齐墩果酸抑制A375黑色素瘤细胞生长并诱导细胞凋亡

V. Cijo George , D.R. Naveen Kumar , P.K. Suresh , R. Ashok Kumar
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引用次数: 11

摘要

黑色素瘤是一种危及生命的疾病,尽管目前的化疗技术取得了进步,但它主要影响的是恶性肿瘤。本研究旨在探讨齐墩果酸(OA)在A375人黑色素瘤细胞中的诱导凋亡潜能。采用细胞生长和XTT法观察OA (12.5 ~ 200 μM)的抗增殖作用。Wright-Giemsa染色和DAPI染色分别进行形态学和核损伤研究。采用DNA片段化ELISA法检测OA诱导A375细胞凋亡的能力。结果显示,OA具有明显的剂量响应效应,可抑制细胞生长(P <0.05),细胞活力降低(XTT数据)。与对照细胞相比,A375细胞发生了显著的形态学变化,包括细胞湮灭。ELISA结果中凋亡dna片段和DAPI结果中核片段的定量剂量依赖性增加进一步证明了该三萜在浓度(特别是高于50 μM)下诱导凋亡细胞死亡的潜力。因此,我们得出结论,OA对A375黑色素瘤细胞具有抗增殖和诱导凋亡的潜力,可能是其进展的更好选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oleanolic acid inhibits cell growth and induces apoptosis in A375 melanoma cells

Melanoma is a life threatening condition, which mostly effects cocassions despite the advancements in current chemotherapeutic techniques. The aim of present study is to investigate the apoptotic inducing potential of oleanolic acid (OA) in A375 human melanoma cells. The anti-proliferative effects of OA (12.5–200 μM) were assessed by cell growth and XTT assay. The morphological and nuclear damage studies were carried out by Wright-Giemsa and DAPI staining, respectively. Further, the apoptotic inducing potential of OA in A375 cells were measured by DNA fragmentation ELISA. The results showed a dose-responsive effect of OA by inhibiting the cell growth significantly (P < 0.05) at 24 and 48 h with a decrease in cell viability (XTT data). The significant morphological changes included cellular annihilation, which was observed in A375 cells when compared to the control cells. Quantitative dose-dependent increase in apoptotic-DNA fragments in ELISA and nuclear fragments in DAPI results, further demonstrated the potential of this triterpenoid to induce apoptotic cell death at a concentration, particularly higher than 50 μM. Thus, we conclude that OA has wielded both anti-proliferative and apoptotic inducing potentials against A375 melanoma cells and can be a better choice for its progression.

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