腹腔注入重组人粒细胞集落刺激因子治疗急性腹膜炎的实验研究

A. I. Shurma, F. Grynchuk
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After 6 hours: in the control, the number of polymorphonuclear leukocytes (PLL) - 2.7±0.39, in the experiment - 3.9±0.38 (р<0.05); in the control, the number of lymphocytes (LC) - 0.2±0.13, in the experiment - 0.7±0.33 (р<0.05). After 12 hours: in the control, the number of PLL - 3.1±0.62, in the experiment - 4.6±0.45 (р<0.05); in the control, the number of LC - 0-1 in the field of vision, in the experiment - 1.8±0.33; in the experiment, the number of fibroblasts (FB) was 0.9±0.27. After 24 hours: in the control, the number of PLL - 3.3±0.39, in the experiment - 1.3±0.33; in the control, the number of LC - 1.8±0.41, in the experiment - 2.3±0.33; in the control, the number of FB - 0.4±0.16, in the experiment - 1.6±0.31 (р<0.01); in the control, the number of macrophages (MF) - 0.4±0.16, in the experiment - 0.7±0.21; in the experiment, the number of plasma cells (PC) was 1.1±0.28. After 48 hours: in the control, the number of PLL - 2.1±0.27, in the experiment - 1.4±0.31 (р<0.05); in the control, the number of LC - 2.2±0.29, in the experiment - 3.3±0.37 (р<0.05); in the control, the number of FB - 1.9±0.34, in the experiment - 2.9±0.23 (р<0.05); in the control, the number of macrophages (MF) – 2.0±0.36, in the experiment – 3.4±0.22 (р<0.01); in the control, the number of PCs was 0.9±0.28, in the experiment - 3.1±0.27 (р<0.01). Conclusions. In animals with AP models, signs of a delay in the local response of immunocompetent cells for 12-24 hours, regeneration processes for 24-48 hours, and slowing down of the regression of the inflammatory process in the peritoneum are observed after the peritoneal cavity is cleaned with a decamethoxine solution. In animals that received intraperitoneal instillation of G-CSF after rehabilitation, signs of early activation of the local reaction of immune cells and regeneration processes are observed, along with the acceleration of the regression of inflammation. The results of the experiments indicate that intraperitoneal instillation of G-CSF can be used under clinical conditions for the treatment of patients with AP.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"32 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Experimental study of intra-peritoneal instilation of recombinant human granulocyte colony-stimulating factor for the treatment of acute peritonitis\",\"authors\":\"A. I. Shurma, F. Grynchuk\",\"doi\":\"10.24061/2413-0737.xxvi.4.104.2022.8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. 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After 6 hours: in the control, the number of polymorphonuclear leukocytes (PLL) - 2.7±0.39, in the experiment - 3.9±0.38 (р<0.05); in the control, the number of lymphocytes (LC) - 0.2±0.13, in the experiment - 0.7±0.33 (р<0.05). After 12 hours: in the control, the number of PLL - 3.1±0.62, in the experiment - 4.6±0.45 (р<0.05); in the control, the number of LC - 0-1 in the field of vision, in the experiment - 1.8±0.33; in the experiment, the number of fibroblasts (FB) was 0.9±0.27. After 24 hours: in the control, the number of PLL - 3.3±0.39, in the experiment - 1.3±0.33; in the control, the number of LC - 1.8±0.41, in the experiment - 2.3±0.33; in the control, the number of FB - 0.4±0.16, in the experiment - 1.6±0.31 (р<0.01); in the control, the number of macrophages (MF) - 0.4±0.16, in the experiment - 0.7±0.21; in the experiment, the number of plasma cells (PC) was 1.1±0.28. After 48 hours: in the control, the number of PLL - 2.1±0.27, in the experiment - 1.4±0.31 (р<0.05); in the control, the number of LC - 2.2±0.29, in the experiment - 3.3±0.37 (р<0.05); in the control, the number of FB - 1.9±0.34, in the experiment - 2.9±0.23 (р<0.05); in the control, the number of macrophages (MF) – 2.0±0.36, in the experiment – 3.4±0.22 (р<0.01); in the control, the number of PCs was 0.9±0.28, in the experiment - 3.1±0.27 (р<0.01). Conclusions. In animals with AP models, signs of a delay in the local response of immunocompetent cells for 12-24 hours, regeneration processes for 24-48 hours, and slowing down of the regression of the inflammatory process in the peritoneum are observed after the peritoneal cavity is cleaned with a decamethoxine solution. 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引用次数: 0

摘要

的目标。本实验探讨重组人粒细胞集落刺激因子(G-CSF)腹腔注射治疗急性腹膜炎(AP)的可能性。材料和方法。60只非线性小白鼠。通过腹腔穿刺20%的自体粪便混合物来建立AP模型。12小时后,进行剖腹手术和腹腔清洁。在30只动物(对照组)中,使用十甲氧辛溶液。实验中,卫生后,以每100 g质量10万单位的剂量将g - csf的NaCl溶液注入腹腔。术后6、12、24、48小时分别行开腹手术,取腹膜顶骨检查。在组织准备的数字拷贝上计数细胞数量。结果。6小时后:对照组多态核白细胞(PLL)数- 2.7±0.39,实验组多态核白细胞数- 3.9±0.38 (p <0.05);对照组淋巴细胞数(LC) - 0.2±0.13,实验组- 0.7±0.33 (p <0.05)。12小时后:对照组PLL数- 3.1±0.62个,实验组PLL数- 4.6±0.45个(p <0.05);对照组中LC - 0-1在视野内的数量,在实验组中为- 1.8±0.33;实验中,成纤维细胞数(FB)为0.9±0.27。24小时后:对照组中,锁相环数- 3.3±0.39,实验组中- 1.3±0.33;对照组中LC数- 1.8±0.41,实验组中LC数- 2.3±0.33;对照组中,FB数为- 0.4±0.16,试验组为- 1.6±0.31 (p <0.01);对照组巨噬细胞数(MF) - 0.4±0.16,实验组巨噬细胞数- 0.7±0.21;实验中,浆细胞数(PC)为1.1±0.28。48h后:对照组PLL数- 2.1±0.27个,实验组PLL数- 1.4±0.31个(p <0.05);对照组中LC数- 2.2±0.29,实验组中LC数- 3.3±0.37 (p <0.05);对照组中,FB数为- 1.9±0.34,试验组为- 2.9±0.23 (p <0.05);对照组巨噬细胞数(MF) - 2.0±0.36,实验组巨噬细胞数- 3.4±0.22 (p <0.01);对照组为0.9±0.28,试验组为- 3.1±0.27 (p <0.01)。结论。在AP模型动物中,用十甲氧辛溶液清洗腹膜腔后,观察到免疫活性细胞的局部反应延迟12-24小时,再生过程延迟24-48小时,腹膜炎症过程消退减慢。在康复后接受G-CSF腹腔灌注的动物中,观察到免疫细胞局部反应和再生过程的早期激活迹象,同时炎症消退加速。实验结果表明,在临床条件下,腹腔注射G-CSF可用于治疗AP患者。
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Experimental study of intra-peritoneal instilation of recombinant human granulocyte colony-stimulating factor for the treatment of acute peritonitis
Aim. In the experiment, the possibility of intraperitoneal application of recombinant human granulocyte colony-stimulating factor (G-CSF) for the treatment of acute peritonitis (AP) was investigated.Materials and methods. 60 non-linear white rats. AP was modeled by intra-abdominal puncture of 20% autofecal mixture. After 12 hours, a laparotomy and sanitation of the peritoneal cavity were performed. In 30 animals (control), a solution of decamethoxine was used. In the experiment, after sanitation, a solution of G-CSF on NaCl was injected into the peritoneal cavity at a dose of 0.1 million units per 100 g of mass. After 6, 12, 24 and 48 hours, a relaparotomy was performed and the parietal peritoneum was taken for examination. The number of cells was counted on digital copies of histological preparations.The results. After 6 hours: in the control, the number of polymorphonuclear leukocytes (PLL) - 2.7±0.39, in the experiment - 3.9±0.38 (р<0.05); in the control, the number of lymphocytes (LC) - 0.2±0.13, in the experiment - 0.7±0.33 (р<0.05). After 12 hours: in the control, the number of PLL - 3.1±0.62, in the experiment - 4.6±0.45 (р<0.05); in the control, the number of LC - 0-1 in the field of vision, in the experiment - 1.8±0.33; in the experiment, the number of fibroblasts (FB) was 0.9±0.27. After 24 hours: in the control, the number of PLL - 3.3±0.39, in the experiment - 1.3±0.33; in the control, the number of LC - 1.8±0.41, in the experiment - 2.3±0.33; in the control, the number of FB - 0.4±0.16, in the experiment - 1.6±0.31 (р<0.01); in the control, the number of macrophages (MF) - 0.4±0.16, in the experiment - 0.7±0.21; in the experiment, the number of plasma cells (PC) was 1.1±0.28. After 48 hours: in the control, the number of PLL - 2.1±0.27, in the experiment - 1.4±0.31 (р<0.05); in the control, the number of LC - 2.2±0.29, in the experiment - 3.3±0.37 (р<0.05); in the control, the number of FB - 1.9±0.34, in the experiment - 2.9±0.23 (р<0.05); in the control, the number of macrophages (MF) – 2.0±0.36, in the experiment – 3.4±0.22 (р<0.01); in the control, the number of PCs was 0.9±0.28, in the experiment - 3.1±0.27 (р<0.01). Conclusions. In animals with AP models, signs of a delay in the local response of immunocompetent cells for 12-24 hours, regeneration processes for 24-48 hours, and slowing down of the regression of the inflammatory process in the peritoneum are observed after the peritoneal cavity is cleaned with a decamethoxine solution. In animals that received intraperitoneal instillation of G-CSF after rehabilitation, signs of early activation of the local reaction of immune cells and regeneration processes are observed, along with the acceleration of the regression of inflammation. The results of the experiments indicate that intraperitoneal instillation of G-CSF can be used under clinical conditions for the treatment of patients with AP.
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