Occlusion of Dentinal Tubules Using a Novel Dentifrice with Fluoro-Hydroxyapatite Nanoparticles Derived From A Biologic Source. (an in vitro study)

Introduction: Dentin hypersensitivity is a widespread issue with high prevalence. Blocking the open dentinal tubules is one way to reduce dentin hypersensitivity. Aim: The current study aimed at evaluating the in vitro potential of a novel experimental dentifrice made of nano-fluoro hydroxyapatite (n-FHAP) produced from a biogenic source (cuttlefishbones) for occlusion of dentinal tubules. Material and Methods: 30 dentin discs (n = 30) were classified into 3 categories (n = 10) based on the dentifrices they utilized for brushing: 1st category = artificial saliva (AS; control); 2nd category = fluoride dentifrice (Sensodyne, UK); 3rd category = experimental nano-FHAP dentifrice. Then, after brushing, discs were subjected to an acidic challenge with 6 percent wt citric acid (pH = 4.0) for 1 minute. By utilizing a SEM (scanning electron microscope) and EDX (energy-dispersive X-ray) spectroscopy before and after the citric acid challenge, the tubule occlusion was measured. Results: Group 1 (AS) SE micrographs revealed no tubule blockage. The tubules in Groups 2 and 3 were mostly blocked, according to SE micrographs. Data before and after the citric acid challenge data were statistically significant (p= 0.05) for all groups. Conclusion: In terms of tubule blockage, the findings of the new experimental dentifrice were equivalent to those of Sensodyne. Dentifrice containing FHAP nano-particles might be used to treat dentin sensitivity as an alternative.