具有可扩展高度和内置储层的水凝胶冲压件，用于在3D拓扑结构上绘制生物分子图案

2007 IEEE 20th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2007-01-01 DOI:10.1109/MEMSYS.2007.4433117

A. Salim, Z. Ding, Babak Ziaie

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引用次数: 0

摘要

在本文中，我们报道了一种具有内置储层的可膨胀高度水凝胶压印机，该压印机可用于在平面和非平面表面上重复(无需频繁重新上墨)印制功能性生物分子。我们成功地将荧光素异硫氰酸酯(FITC)标记的抗体(兔制备的缓生根瘤菌o抗原抗体)在平面(硅)和非平面(SU8在硅上，高度约为70 μ m)拓扑结构上进行了图图化。打印的抗体显示了水凝胶压印器在3D拓扑结构上传递生物分子的有效性，其中水凝胶可以调整以压印在深沟槽的侧壁和底部。

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A hydrogel stamper with expandable height and built-in reservoirs for patterning biomolecules on 3D topologies

In this paper, we report on an expandable height hydrogel stamper with built-in reservoir that can be used to repeatedly (without frequent re-inking) stamp functional biomolecules on planar and non-planar surfaces. We have successfully patterned fluorescein isothiocyante (FITC) labeled antibodies (antibodies for O-antigens of Bradyrhizobium japonicum made in rabbits) on planar (silicon) and nonplanar (SU8 on silicon with a height of ~70 mum) topologies. The printed antibodies show the effectiveness of hydrogel stampers in delivering biomolecules on 3D topologies where the hydrogel could be adjusted to stamp on the sidewalls and bottom of deep trenches.

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来源期刊

2007 IEEE 20th International Conference on Micro Electro Mechanical Systems (MEMS)

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