Ibraheem M. Attafi, Magbool E. Oraiby, Mohsen M. Fageeh, Ghassam A. Shaikhain, Mohammed Y. Albeishy, Ibrahim A. Khardali, Mohammed A. Hakami
{"title":"致死性摄入二嗪农病例中二嗪农及其主要代谢物2-异丙基-4-甲基-6-羟基嘧啶的尸检分析","authors":"Ibraheem M. Attafi, Magbool E. Oraiby, Mohsen M. Fageeh, Ghassam A. Shaikhain, Mohammed Y. Albeishy, Ibrahim A. Khardali, Mohammed A. Hakami","doi":"10.26735/16586794.2018.033","DOIUrl":null,"url":null,"abstract":"This case report describes a detection and quantitation method for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP), in postmortem blood and tissue samples from a fatal case of diazinon ingestion. Diazinon and IMHP were extracted from postmortem samples with a liquid/liquid method and analyzed by gas chromatography mass spectrometry (GC/MS). By comparing to diazinon standard and matching the retention time, diazinon was detected in two visceral organs, the stomach (0.89 μg/g) and the small intestine (8.80 μg/g). The highest level of diazinon was detected in the small intestine (8.80 μg/g), whereas the highest amount of IMHP was noted in the kidney (0.84 Area %) and bladder (0.75 Area %). In conclusion, determination of IMHP in postmortem samples could be used as an indicator for diazinon exposure, especially in the case of delayed death; whereas, the small intestine could be the best source of sample in diazinon assessment in cases of fatal diazinon ingestion.","PeriodicalId":31692,"journal":{"name":"Arab Journal of Forensic Sciences Forensic Medicine","volume":"380 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Post-Mortem Analysis of Diazinon and its major Metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine, in a Case of Fatal Diazinon Ingestion\",\"authors\":\"Ibraheem M. Attafi, Magbool E. Oraiby, Mohsen M. Fageeh, Ghassam A. Shaikhain, Mohammed Y. Albeishy, Ibrahim A. Khardali, Mohammed A. Hakami\",\"doi\":\"10.26735/16586794.2018.033\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This case report describes a detection and quantitation method for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP), in postmortem blood and tissue samples from a fatal case of diazinon ingestion. Diazinon and IMHP were extracted from postmortem samples with a liquid/liquid method and analyzed by gas chromatography mass spectrometry (GC/MS). By comparing to diazinon standard and matching the retention time, diazinon was detected in two visceral organs, the stomach (0.89 μg/g) and the small intestine (8.80 μg/g). The highest level of diazinon was detected in the small intestine (8.80 μg/g), whereas the highest amount of IMHP was noted in the kidney (0.84 Area %) and bladder (0.75 Area %). In conclusion, determination of IMHP in postmortem samples could be used as an indicator for diazinon exposure, especially in the case of delayed death; whereas, the small intestine could be the best source of sample in diazinon assessment in cases of fatal diazinon ingestion.\",\"PeriodicalId\":31692,\"journal\":{\"name\":\"Arab Journal of Forensic Sciences Forensic Medicine\",\"volume\":\"380 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Arab Journal of Forensic Sciences Forensic Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26735/16586794.2018.033\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Social Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arab Journal of Forensic Sciences Forensic Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26735/16586794.2018.033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Social Sciences","Score":null,"Total":0}
Post-Mortem Analysis of Diazinon and its major Metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine, in a Case of Fatal Diazinon Ingestion
This case report describes a detection and quantitation method for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP), in postmortem blood and tissue samples from a fatal case of diazinon ingestion. Diazinon and IMHP were extracted from postmortem samples with a liquid/liquid method and analyzed by gas chromatography mass spectrometry (GC/MS). By comparing to diazinon standard and matching the retention time, diazinon was detected in two visceral organs, the stomach (0.89 μg/g) and the small intestine (8.80 μg/g). The highest level of diazinon was detected in the small intestine (8.80 μg/g), whereas the highest amount of IMHP was noted in the kidney (0.84 Area %) and bladder (0.75 Area %). In conclusion, determination of IMHP in postmortem samples could be used as an indicator for diazinon exposure, especially in the case of delayed death; whereas, the small intestine could be the best source of sample in diazinon assessment in cases of fatal diazinon ingestion.